CRISPR-U™ Gene Knockout Cell Line Strategy

DNAH5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DNAH5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DNAH5
Gene id 1767
Organism Homo sapiens
Official full symbol dynein axonemal heavy chain 5
Gene type protein-coding
Also known as CILD3, DNAHC5, HL1, KTGNR, PCD
Summary This gene encodes a dynein protein, which is part of a microtubule-associated motor protein complex consisting of heavy, light, and intermediate chains. This protein is an axonemal heavy chain dynein. It functions as a force-generating protein with ATPase activity, whereby the release of ADP is thought to produce the force-producing power stroke. Mutations in this gene cause primary ciliary dyskinesia type 3, as well as Kartagener syndrome, which are both diseases due to ciliary defects.
Genomic regions Chromosome 5
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DNAH5-201 ENST00000265104.5 15781 4624aa Protein coding CCDS3882 Q8TE73 NM_001369.3 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
DNAH5-206 ENST00000681290.1 15645 4609aa Protein coding - - - GENCODE basic, APPRIS ALT2,
DNAH5-205 ENST00000680213.1 1730 478aa Non stop decay - - - -
DNAH5-203 ENST00000508040.1 2081 205aa Nonsense mediated decay - - - TSL:2,
DNAH5-202 ENST00000504001.3 760 No protein Processed transcript - - - TSL:5,
DNAH5-204 ENST00000512443.1 676 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DNAH5
This KO Strategy loading
Red Cotton™ Notes Gene DNAH5 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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