CRISPR-U™ Gene Knockout Cell Line Strategy

ASXL1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ASXL1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ASXL1
Gene id 171023
Organism Homo sapiens
Official full symbol ASXL transcriptional regulator 1
Gene type protein-coding
Also known as BOPS, MDS
Summary This gene is similar to the Drosophila additional sex combs gene, which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 20
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ASXL1-202 ENST00000375687.10 7052 1541aa Protein coding CCDS13201 Q8IXJ9-1 NM_015338.6 TSL:5, GENCODE basic, APPRIS P2, MANE Select v0.92,
ASXL1-224 ENST00000646985.1 6666 1480aa Protein coding CCDS86945 A0A2R8Y5U1 - GENCODE basic, APPRIS ALT2,
ASXL1-223 ENST00000646367.1 1065 85aa Protein coding CCDS86944 Q498B9 - GENCODE basic,
ASXL1-210 ENST00000613218.4 7038 1541aa Protein coding - Q8IXJ9-1 - TSL:5, GENCODE basic, APPRIS P2,
ASXL1-201 ENST00000306058.9 6591 1536aa Protein coding - Q76L82 - TSL:1, GENCODE basic, APPRIS ALT2,
ASXL1-212 ENST00000620121.4 5374 1541aa Protein coding - Q8IXJ9-1 - TSL:5, GENCODE basic, APPRIS P2,
ASXL1-227 ENST00000651418.1 3146 625aa Protein coding - A0A494C1R1 - GENCODE basic,
ASXL1-206 ENST00000542461.5 1068 85aa Protein coding - Q498B9 - TSL:5, GENCODE basic,
ASXL1-214 ENST00000643168.1 840 135aa Protein coding - A0A2R8Y627 - CDS 3' incomplete,
ASXL1-203 ENST00000375689.5 812 81aa Protein coding - Q5JWS8 - TSL:5, GENCODE basic,
ASXL1-222 ENST00000645688.1 500 78aa Protein coding - A0A2R8Y6V0 - CDS 3' incomplete,
ASXL1-205 ENST00000497249.6 495 75aa Protein coding - H0YJC9 - TSL:5, GENCODE basic,
ASXL1-226 ENST00000649901.1 1261 57aa Nonsense mediated decay - A0A087WWN0 - -
ASXL1-217 ENST00000644587.1 1076 84aa Nonsense mediated decay - Q6P1M8 - -
ASXL1-208 ENST00000555343.2 1034 57aa Nonsense mediated decay - A0A087WWN0 - TSL:5,
ASXL1-220 ENST00000645337.1 642 85aa Nonsense mediated decay - A0A2R8Y4U9 - -
ASXL1-219 ENST00000645035.1 609 75aa Nonsense mediated decay - A0A2R8Y6F0 - -
ASXL1-213 ENST00000642676.1 535 57aa Nonsense mediated decay - A0A087WWN0 - -
ASXL1-207 ENST00000553345.5 809 No protein Processed transcript - - - TSL:5,
ASXL1-225 ENST00000647223.1 8056 No protein Retained intron - - - -
ASXL1-216 ENST00000644168.1 1466 No protein Retained intron - - - -
ASXL1-215 ENST00000644060.1 1202 No protein Retained intron - - - -
ASXL1-204 ENST00000470145.3 1085 No protein Retained intron - - - TSL:5,
ASXL1-211 ENST00000619344.4 550 No protein Retained intron - - - TSL:5,
ASXL1-209 ENST00000555564.2 449 No protein Retained intron - - - TSL:4,
ASXL1-221 ENST00000645514.1 392 No protein Retained intron - - - -
ASXL1-218 ENST00000644615.1 355 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ASXL1
This KO Strategy loading
Red Cotton™ Notes Gene ASXL1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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