CRISPR-U™ Gene Knockout Cell Line
Strategy
ASXL1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ASXL1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ASXL1 |
| Gene id | 171023 |
| Organism | Homo sapiens |
| Official full symbol | ASXL transcriptional regulator 1 |
| Gene type | protein-coding |
| Also known as | BOPS, MDS |
| Summary | This gene is similar to the Drosophila additional sex combs gene, which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ASXL1-202 | ENST00000375687.10 | 7052 | 1541aa | Protein coding | CCDS13201 | Q8IXJ9-1 | NM_015338.6 | TSL:5, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| ASXL1-224 | ENST00000646985.1 | 6666 | 1480aa | Protein coding | CCDS86945 | A0A2R8Y5U1 | - | GENCODE basic, APPRIS ALT2, |
| ASXL1-223 | ENST00000646367.1 | 1065 | 85aa | Protein coding | CCDS86944 | Q498B9 | - | GENCODE basic, |
| ASXL1-210 | ENST00000613218.4 | 7038 | 1541aa | Protein coding | - | Q8IXJ9-1 | - | TSL:5, GENCODE basic, APPRIS P2, |
| ASXL1-201 | ENST00000306058.9 | 6591 | 1536aa | Protein coding | - | Q76L82 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ASXL1-212 | ENST00000620121.4 | 5374 | 1541aa | Protein coding | - | Q8IXJ9-1 | - | TSL:5, GENCODE basic, APPRIS P2, |
| ASXL1-227 | ENST00000651418.1 | 3146 | 625aa | Protein coding | - | A0A494C1R1 | - | GENCODE basic, |
| ASXL1-206 | ENST00000542461.5 | 1068 | 85aa | Protein coding | - | Q498B9 | - | TSL:5, GENCODE basic, |
| ASXL1-214 | ENST00000643168.1 | 840 | 135aa | Protein coding | - | A0A2R8Y627 | - | CDS 3' incomplete, |
| ASXL1-203 | ENST00000375689.5 | 812 | 81aa | Protein coding | - | Q5JWS8 | - | TSL:5, GENCODE basic, |
| ASXL1-222 | ENST00000645688.1 | 500 | 78aa | Protein coding | - | A0A2R8Y6V0 | - | CDS 3' incomplete, |
| ASXL1-205 | ENST00000497249.6 | 495 | 75aa | Protein coding | - | H0YJC9 | - | TSL:5, GENCODE basic, |
| ASXL1-226 | ENST00000649901.1 | 1261 | 57aa | Nonsense mediated decay | - | A0A087WWN0 | - | - |
| ASXL1-217 | ENST00000644587.1 | 1076 | 84aa | Nonsense mediated decay | - | Q6P1M8 | - | - |
| ASXL1-208 | ENST00000555343.2 | 1034 | 57aa | Nonsense mediated decay | - | A0A087WWN0 | - | TSL:5, |
| ASXL1-220 | ENST00000645337.1 | 642 | 85aa | Nonsense mediated decay | - | A0A2R8Y4U9 | - | - |
| ASXL1-219 | ENST00000645035.1 | 609 | 75aa | Nonsense mediated decay | - | A0A2R8Y6F0 | - | - |
| ASXL1-213 | ENST00000642676.1 | 535 | 57aa | Nonsense mediated decay | - | A0A087WWN0 | - | - |
| ASXL1-207 | ENST00000553345.5 | 809 | No protein | Processed transcript | - | - | - | TSL:5, |
| ASXL1-225 | ENST00000647223.1 | 8056 | No protein | Retained intron | - | - | - | - |
| ASXL1-216 | ENST00000644168.1 | 1466 | No protein | Retained intron | - | - | - | - |
| ASXL1-215 | ENST00000644060.1 | 1202 | No protein | Retained intron | - | - | - | - |
| ASXL1-204 | ENST00000470145.3 | 1085 | No protein | Retained intron | - | - | - | TSL:5, |
| ASXL1-211 | ENST00000619344.4 | 550 | No protein | Retained intron | - | - | - | TSL:5, |
| ASXL1-209 | ENST00000555564.2 | 449 | No protein | Retained intron | - | - | - | TSL:4, |
| ASXL1-221 | ENST00000645514.1 | 392 | No protein | Retained intron | - | - | - | - |
| ASXL1-218 | ENST00000644615.1 | 355 | No protein | Retained intron | - | - | - | - |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ASXL1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ASXL1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
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$599
Single gRNA lentivirus
Work flow
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