CRISPR-U™ Gene Knockout Cell Line Strategy

IDO2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IDO2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IDO2
Gene id 169355
Organism Homo sapiens
Official full symbol indoleamine 2,3-dioxygenase 2
Gene type protein-coding
Also known as INDOL1
Summary Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 ).
Genomic regions Chromosome 8
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IDO2-204 ENST00000502986.2 2294 420aa Protein coding CCDS6114 Q6ZQW0-1 - TSL:5, GENCODE basic, APPRIS P4,
IDO2-202 ENST00000389060.8 1227 407aa Protein coding - A0A0N4SWI6 - TSL:1, GENCODE basic, APPRIS ALT2,
IDO2-201 ENST00000343295.8 4264 No protein Processed transcript - - - TSL:2,
IDO2-203 ENST00000418094.1 1640 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IDO2
This KO Strategy loading
Red Cotton™ Notes Gene IDO2 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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