CRISPR-U™ Gene Knockout Cell Line
Strategy
IDO2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IDO2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IDO2 |
| Gene id | 169355 |
| Organism | Homo sapiens |
| Official full symbol | indoleamine 2,3-dioxygenase 2 |
| Gene type | protein-coding |
| Also known as | INDOL1 |
| Summary | Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 ). |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IDO2-204 | ENST00000502986.2 | 2294 | 420aa | Protein coding | CCDS6114 | Q6ZQW0-1 | - | TSL:5, GENCODE basic, APPRIS P4, |
| IDO2-202 | ENST00000389060.8 | 1227 | 407aa | Protein coding | - | A0A0N4SWI6 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IDO2-201 | ENST00000343295.8 | 4264 | No protein | Processed transcript | - | - | - | TSL:2, |
| IDO2-203 | ENST00000418094.1 | 1640 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IDO2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IDO2 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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