CRISPR-U™ Gene Knockout Cell Line Strategy

Lgals9 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human Lgals9 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol Lgals9
Gene id 16859
Organism Mus musculus
Official full symbol lectin, galactose binding, soluble 9
Gene type protein-coding
Also known as AA407335, AI194909, AI265545, LGALS35, Lgals5, gal-9, galectin-9
Genomic regions Chromosome 11
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match Flags
Lgals9-203 ENSMUST00000108269.10 1586 353aa Protein coding CCDS25116 G3X9T7 TSL:1, GENCODE basic, APPRIS P3,
Lgals9-202 ENSMUST00000108268.10 1520 322aa Protein coding CCDS48858 O08573-2 TSL:1, GENCODE basic, APPRIS ALT2,
Lgals9-201 ENSMUST00000073001.5 1074 352aa Protein coding - B1AQR8 TSL:5, GENCODE basic, APPRIS ALT2,
Lgals9-204 ENSMUST00000140073.2 742 No protein Retained intron - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene Lgals9
This KO Strategy loading
Red Cotton™ Notes Gene Lgals9 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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