CRISPR-U™ Gene Knockout Cell Line Strategy

Arhgef1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human Arhgef1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol Arhgef1
Gene id 16801
Organism Mus musculus
Official full symbol Rho guanine nucleotide exchange factor (GEF) 1
Gene type protein-coding
Also known as Lbcl2, Lsc
Genomic regions Chromosome 7
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match Flags
Arhgef1-204 ENSMUST00000117796.8 3508 976aa Protein coding CCDS52140 E9PUF7 TSL:1, GENCODE basic, APPRIS ALT2,
Arhgef1-202 ENSMUST00000098683.11 3375 979aa Protein coding CCDS52142 Q61210-5 TSL:1, GENCODE basic, APPRIS P4,
Arhgef1-203 ENSMUST00000117419.8 3214 920aa Protein coding CCDS52141 Q61210-1 TSL:1, GENCODE basic, APPRIS ALT2,
Arhgef1-201 ENSMUST00000047873.16 3212 920aa Protein coding CCDS52141 Q61210-1 TSL:1, GENCODE basic, APPRIS ALT2,
Arhgef1-218 ENSMUST00000206508.2 3195 919aa Protein coding CCDS85239 Q61210-2 TSL:1, GENCODE basic, APPRIS ALT2,
Arhgef1-210 ENSMUST00000132751.2 1851 616aa Protein coding - F6ZN61 CDS 5' incomplete, TSL:5,
Arhgef1-215 ENSMUST00000205295.2 777 233aa Protein coding - A0A0U1RPN7 CDS 3' incomplete, TSL:5,
Arhgef1-219 ENSMUST00000206906.2 737 192aa Protein coding - A0A0U1RPP2 CDS 3' incomplete, TSL:5,
Arhgef1-216 ENSMUST00000206011.2 419 102aa Protein coding - A0A0U1RPH2 CDS 3' incomplete, TSL:2,
Arhgef1-214 ENSMUST00000151121.2 390 101aa Protein coding - D3YVJ9 CDS 3' incomplete, TSL:3,
Arhgef1-217 ENSMUST00000206028.2 374 30aa Protein coding - A0A0U1RNQ2 CDS 3' incomplete, TSL:3,
Arhgef1-211 ENSMUST00000132786.2 832 No protein Processed transcript - - TSL:5,
Arhgef1-208 ENSMUST00000129383.2 351 No protein Processed transcript - - TSL:2,
Arhgef1-213 ENSMUST00000145783.8 4243 No protein Retained intron - - TSL:5,
Arhgef1-207 ENSMUST00000127761.8 1827 No protein Retained intron - - TSL:2,
Arhgef1-212 ENSMUST00000144714.2 1087 No protein Retained intron - - TSL:3,
Arhgef1-206 ENSMUST00000126918.8 821 No protein Retained intron - - TSL:5,
Arhgef1-209 ENSMUST00000129928.2 777 No protein Retained intron - - TSL:2,
Arhgef1-205 ENSMUST00000126484.2 775 No protein Retained intron - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene Arhgef1
This KO Strategy loading
Red Cotton™ Notes Gene Arhgef1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×