CRISPR-U™ Gene Knockout Cell Line
Strategy
Arhgef1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human Arhgef1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | Arhgef1 |
| Gene id | 16801 |
| Organism | Mus musculus |
| Official full symbol | Rho guanine nucleotide exchange factor (GEF) 1 |
| Gene type | protein-coding |
| Also known as | Lbcl2, Lsc |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | Flags |
| Arhgef1-204 | ENSMUST00000117796.8 | 3508 | 976aa | Protein coding | CCDS52140 | E9PUF7 | TSL:1, GENCODE basic, APPRIS ALT2, |
| Arhgef1-202 | ENSMUST00000098683.11 | 3375 | 979aa | Protein coding | CCDS52142 | Q61210-5 | TSL:1, GENCODE basic, APPRIS P4, |
| Arhgef1-203 | ENSMUST00000117419.8 | 3214 | 920aa | Protein coding | CCDS52141 | Q61210-1 | TSL:1, GENCODE basic, APPRIS ALT2, |
| Arhgef1-201 | ENSMUST00000047873.16 | 3212 | 920aa | Protein coding | CCDS52141 | Q61210-1 | TSL:1, GENCODE basic, APPRIS ALT2, |
| Arhgef1-218 | ENSMUST00000206508.2 | 3195 | 919aa | Protein coding | CCDS85239 | Q61210-2 | TSL:1, GENCODE basic, APPRIS ALT2, |
| Arhgef1-210 | ENSMUST00000132751.2 | 1851 | 616aa | Protein coding | - | F6ZN61 | CDS 5' incomplete, TSL:5, |
| Arhgef1-215 | ENSMUST00000205295.2 | 777 | 233aa | Protein coding | - | A0A0U1RPN7 | CDS 3' incomplete, TSL:5, |
| Arhgef1-219 | ENSMUST00000206906.2 | 737 | 192aa | Protein coding | - | A0A0U1RPP2 | CDS 3' incomplete, TSL:5, |
| Arhgef1-216 | ENSMUST00000206011.2 | 419 | 102aa | Protein coding | - | A0A0U1RPH2 | CDS 3' incomplete, TSL:2, |
| Arhgef1-214 | ENSMUST00000151121.2 | 390 | 101aa | Protein coding | - | D3YVJ9 | CDS 3' incomplete, TSL:3, |
| Arhgef1-217 | ENSMUST00000206028.2 | 374 | 30aa | Protein coding | - | A0A0U1RNQ2 | CDS 3' incomplete, TSL:3, |
| Arhgef1-211 | ENSMUST00000132786.2 | 832 | No protein | Processed transcript | - | - | TSL:5, |
| Arhgef1-208 | ENSMUST00000129383.2 | 351 | No protein | Processed transcript | - | - | TSL:2, |
| Arhgef1-213 | ENSMUST00000145783.8 | 4243 | No protein | Retained intron | - | - | TSL:5, |
| Arhgef1-207 | ENSMUST00000127761.8 | 1827 | No protein | Retained intron | - | - | TSL:2, |
| Arhgef1-212 | ENSMUST00000144714.2 | 1087 | No protein | Retained intron | - | - | TSL:3, |
| Arhgef1-206 | ENSMUST00000126918.8 | 821 | No protein | Retained intron | - | - | TSL:5, |
| Arhgef1-209 | ENSMUST00000129928.2 | 777 | No protein | Retained intron | - | - | TSL:2, |
| Arhgef1-205 | ENSMUST00000126484.2 | 775 | No protein | Retained intron | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | Arhgef1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene Arhgef1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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