CRISPR-U™ Gene Knockout Cell Line Strategy

DDIT3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DDIT3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DDIT3
Gene id 1649
Organism Homo sapiens
Official full symbol DNA damage inducible transcript 3
Gene type protein-coding
Also known as AltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10, CHOP10, GADD153
Summary This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
Genomic regions Chromosome 12
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DDIT3-204 ENST00000551116.5 1067 192aa Protein coding CCDS55838 P35638-2 - TSL:1, GENCODE basic,
DDIT3-205 ENST00000552740.5 951 192aa Protein coding CCDS55838 P35638-2 - TSL:1, GENCODE basic,
DDIT3-206 ENST00000623876.2 940 169aa Protein coding CCDS8943 P35638-1 - TSL:NA, GENCODE basic, APPRIS P1,
DDIT3-201 ENST00000346473.7 906 169aa Protein coding CCDS8943 P35638-1 - TSL:1, GENCODE basic, APPRIS P1,
DDIT3-202 ENST00000547303.5 872 169aa Protein coding CCDS8943 P35638-1 - TSL:2, GENCODE basic, APPRIS P1,
DDIT3-203 ENST00000547526.1 644 145aa Protein coding - F8W133 - CDS 3' incomplete, TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DDIT3
This KO Strategy loading
Red Cotton™ Notes Gene DDIT3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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