CRISPR-U™ Gene Knockout Cell Line
Strategy
DDIT3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DDIT3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DDIT3 |
| Gene id | 1649 |
| Organism | Homo sapiens |
| Official full symbol | DNA damage inducible transcript 3 |
| Gene type | protein-coding |
| Also known as | AltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10, CHOP10, GADD153 |
| Summary | This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DDIT3-204 | ENST00000551116.5 | 1067 | 192aa | Protein coding | CCDS55838 | P35638-2 | - | TSL:1, GENCODE basic, |
| DDIT3-205 | ENST00000552740.5 | 951 | 192aa | Protein coding | CCDS55838 | P35638-2 | - | TSL:1, GENCODE basic, |
| DDIT3-206 | ENST00000623876.2 | 940 | 169aa | Protein coding | CCDS8943 | P35638-1 | - | TSL:NA, GENCODE basic, APPRIS P1, |
| DDIT3-201 | ENST00000346473.7 | 906 | 169aa | Protein coding | CCDS8943 | P35638-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| DDIT3-202 | ENST00000547303.5 | 872 | 169aa | Protein coding | CCDS8943 | P35638-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| DDIT3-203 | ENST00000547526.1 | 644 | 145aa | Protein coding | - | F8W133 | - | CDS 3' incomplete, TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DDIT3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DDIT3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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