CRISPR-U™ Gene Knockout Cell Line Strategy

DDB2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DDB2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DDB2
Gene id 1643
Organism Homo sapiens
Official full symbol damage specific DNA binding protein 2
Gene type protein-coding
Also known as DDBB, UV-DDB2, XPE
Summary This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 11
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DDB2-201 ENST00000256996.9 1815 427aa Protein coding CCDS7927 Q92466-1 NM_000107.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DDB2-202 ENST00000378600.7 717 238aa Protein coding CCDS73284 Q92466-2 - TSL:1, GENCODE basic,
DDB2-204 ENST00000378603.7 1092 363aa Protein coding - Q92466-4 - TSL:1, GENCODE basic,
DDB2-208 ENST00000614825.4 852 227aa Protein coding - A0A087X0X5 - CDS 3' incomplete, TSL:3,
DDB2-215 ENST00000622090.4 580 162aa Protein coding - A0A087WW71 - CDS 3' incomplete, TSL:5,
DDB2-216 ENST00000622878.4 556 93aa Protein coding - A0A087WV56 - CDS 3' incomplete, TSL:4,
DDB2-213 ENST00000617847.4 1649 95aa Nonsense mediated decay - A0A087WYT8 - CDS 5' incomplete, TSL:5,
DDB2-203 ENST00000378601.7 1591 244aa Nonsense mediated decay - Q92466-5 - TSL:2,
DDB2-211 ENST00000616278.4 960 156aa Nonsense mediated decay - Q92466-3 - TSL:2,
DDB2-205 ENST00000610805.4 793 91aa Nonsense mediated decay - A0A087WTQ7 - TSL:5,
DDB2-214 ENST00000620515.1 347 No protein Processed transcript - - - TSL:3,
DDB2-206 ENST00000612309.4 3101 No protein Retained intron - - - TSL:2,
DDB2-212 ENST00000617022.4 2181 No protein Retained intron - - - TSL:2,
DDB2-207 ENST00000614394.1 1088 No protein Retained intron - - - TSL:2,
DDB2-210 ENST00000615695.1 897 No protein Retained intron - - - TSL:2,
DDB2-209 ENST00000614884.1 660 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DDB2
This KO Strategy loading
Red Cotton™ Notes Gene DDB2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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