CRISPR-U™ Gene Knockout Cell Line Strategy

DBI Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DBI Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DBI
Gene id 1622
Organism Homo sapiens
Official full symbol diazepam binding inhibitor, acyl-CoA binding protein
Gene type protein-coding
Also known as ACBD1, ACBP, CCK-RP, EP
Summary This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DBI-209 ENST00000535757.5 740 104aa Protein coding CCDS2126 P07108-2 - TSL:1, GENCODE basic,
DBI-201 ENST00000311521.8 714 104aa Protein coding CCDS2126 P07108-2 - TSL:1, GENCODE basic,
DBI-208 ENST00000535617.5 654 97aa Protein coding CCDS74568 B8ZWD1 - TSL:1, GENCODE basic,
DBI-202 ENST00000355857.7 637 87aa Protein coding CCDS42740 P07108-1 - TSL:1, GENCODE basic, APPRIS P1,
DBI-212 ENST00000627305.2 620 148aa Protein coding CCDS54391 P07108-5 - TSL:1, GENCODE basic,
DBI-203 ENST00000393103.2 599 88aa Protein coding CCDS42741 P07108-3 - TSL:1, GENCODE basic,
DBI-204 ENST00000409094.5 576 104aa Protein coding CCDS2126 P07108-2 - TSL:1, GENCODE basic,
DBI-211 ENST00000627093.2 554 129aa Protein coding CCDS54390 P07108-4 - TSL:1, GENCODE basic,
DBI-210 ENST00000542275.5 757 143aa Protein coding - A0A0A0MTI5 - TSL:5, GENCODE basic,
DBI-205 ENST00000460901.1 774 No protein Processed transcript - - - TSL:1,
DBI-206 ENST00000475783.1 842 No protein Retained intron - - - TSL:1,
DBI-207 ENST00000492375.1 784 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DBI
This KO Strategy loading
Red Cotton™ Notes Gene DBI had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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