CRISPR-U™ Gene Knockout Cell Line
Strategy
DBI Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DBI Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DBI |
| Gene id | 1622 |
| Organism | Homo sapiens |
| Official full symbol | diazepam binding inhibitor, acyl-CoA binding protein |
| Gene type | protein-coding |
| Also known as | ACBD1, ACBP, CCK-RP, EP |
| Summary | This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DBI-209 | ENST00000535757.5 | 740 | 104aa | Protein coding | CCDS2126 | P07108-2 | - | TSL:1, GENCODE basic, |
| DBI-201 | ENST00000311521.8 | 714 | 104aa | Protein coding | CCDS2126 | P07108-2 | - | TSL:1, GENCODE basic, |
| DBI-208 | ENST00000535617.5 | 654 | 97aa | Protein coding | CCDS74568 | B8ZWD1 | - | TSL:1, GENCODE basic, |
| DBI-202 | ENST00000355857.7 | 637 | 87aa | Protein coding | CCDS42740 | P07108-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| DBI-212 | ENST00000627305.2 | 620 | 148aa | Protein coding | CCDS54391 | P07108-5 | - | TSL:1, GENCODE basic, |
| DBI-203 | ENST00000393103.2 | 599 | 88aa | Protein coding | CCDS42741 | P07108-3 | - | TSL:1, GENCODE basic, |
| DBI-204 | ENST00000409094.5 | 576 | 104aa | Protein coding | CCDS2126 | P07108-2 | - | TSL:1, GENCODE basic, |
| DBI-211 | ENST00000627093.2 | 554 | 129aa | Protein coding | CCDS54390 | P07108-4 | - | TSL:1, GENCODE basic, |
| DBI-210 | ENST00000542275.5 | 757 | 143aa | Protein coding | - | A0A0A0MTI5 | - | TSL:5, GENCODE basic, |
| DBI-205 | ENST00000460901.1 | 774 | No protein | Processed transcript | - | - | - | TSL:1, |
| DBI-206 | ENST00000475783.1 | 842 | No protein | Retained intron | - | - | - | TSL:1, |
| DBI-207 | ENST00000492375.1 | 784 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DBI |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DBI had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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