CRISPR-U™ Gene Knockout Cell Line
Strategy
CYP1B1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CYP1B1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CYP1B1 |
| Gene id | 1545 |
| Organism | Homo sapiens |
| Official full symbol | cytochrome P450 family 1 subfamily B member 1 |
| Gene type | protein-coding |
| Also known as | ASGD6, CP1B, CYPIB1, GLC3A, P4501B1 |
| Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CYP1B1-205 | ENST00000610745.5 | 5218 | 543aa | Protein coding | CCDS1793 | Q16678 | NM_000104.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CYP1B1-207 | ENST00000614273.1 | 2174 | 543aa | Protein coding | CCDS1793 | Q16678 | - | TSL:5, GENCODE basic, APPRIS P1, |
| CYP1B1-204 | ENST00000494864.1 | 1479 | 172aa | Protein coding | - | A0A087WW26 | - | TSL:5, GENCODE basic, |
| CYP1B1-201 | ENST00000490576.1 | 567 | 143aa | Protein coding | - | A0A087WUQ7 | - | CDS 3' incomplete, TSL:4, |
| CYP1B1-203 | ENST00000492443.1 | 745 | No protein | Processed transcript | - | - | - | TSL:3, |
| CYP1B1-206 | ENST00000613082.1 | 573 | No protein | Processed transcript | - | - | - | TSL:2, |
| CYP1B1-202 | ENST00000491456.1 | 289 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CYP1B1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CYP1B1 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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