CRISPR-U™ Gene Knockout Cell Line Strategy

CYP1B1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CYP1B1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CYP1B1
Gene id 1545
Organism Homo sapiens
Official full symbol cytochrome P450 family 1 subfamily B member 1
Gene type protein-coding
Also known as ASGD6, CP1B, CYPIB1, GLC3A, P4501B1
Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.
Genomic regions Chromosome 2
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CYP1B1-205 ENST00000610745.5 5218 543aa Protein coding CCDS1793 Q16678 NM_000104.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CYP1B1-207 ENST00000614273.1 2174 543aa Protein coding CCDS1793 Q16678 - TSL:5, GENCODE basic, APPRIS P1,
CYP1B1-204 ENST00000494864.1 1479 172aa Protein coding - A0A087WW26 - TSL:5, GENCODE basic,
CYP1B1-201 ENST00000490576.1 567 143aa Protein coding - A0A087WUQ7 - CDS 3' incomplete, TSL:4,
CYP1B1-203 ENST00000492443.1 745 No protein Processed transcript - - - TSL:3,
CYP1B1-206 ENST00000613082.1 573 No protein Processed transcript - - - TSL:2,
CYP1B1-202 ENST00000491456.1 289 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CYP1B1
This KO Strategy loading
Red Cotton™ Notes Gene CYP1B1 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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