CRISPR-U™ Gene Knockout Cell Line
Strategy
CNKSR3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CNKSR3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CNKSR3 |
| Gene id | 154043 |
| Organism | Homo sapiens |
| Official full symbol | CNKSR family member 3 |
| Gene type | protein-coding |
| Also known as | CNK3, CNK3/IPCEF1, MAGI1 |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CNKSR3-205 | ENST00000607772.6 | 21078 | 555aa | Protein coding | CCDS5246 | Q6P9H4-1 | NM_173515.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CNKSR3-204 | ENST00000479339.5 | 2023 | 475aa | Protein coding | - | A0A5H1ZRR2 | - | TSL:1, GENCODE basic, |
| CNKSR3-201 | ENST00000424998.3 | 536 | 178aa | Protein coding | - | Q5T4Y8 | - | CDS 5' and 3' incomplete, TSL:5, |
| CNKSR3-202 | ENST00000433165.6 | 2347 | No protein | Processed transcript | - | - | - | TSL:1, |
| CNKSR3-203 | ENST00000454664.2 | 776 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CNKSR3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CNKSR3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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