CRISPR-U™ Gene Knockout Cell Line Strategy

ADRB1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ADRB1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ADRB1
Gene id 153
Organism Homo sapiens
Official full symbol adrenoceptor beta 1
Gene type protein-coding
Also known as ADRB1R, B1AR, BETA1AR, FNSS2, RHR
Summary The adrenergic receptors (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. Beta-1 adrenoceptors are predominately located in the heart. Specific polymorphisms in this gene have been shown to affect the resting heart rate and can be involved in heart failure.
Genomic regions Chromosome 10
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ADRB1-201 ENST00000369295.4 3039 477aa Protein coding CCDS7586 P08588 NM_000684.3 TSL:NA, GENCODE basic, APPRIS P1, MANE Select v0.92,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ADRB1
This KO Strategy loading
Red Cotton™ Notes Gene ADRB1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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