CRISPR-U™ Gene Knockout Cell Line
Strategy
PPM1K Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPM1K Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPM1K |
| Gene id | 152926 |
| Organism | Homo sapiens |
| Official full symbol | protein phosphatase, Mg2+/Mn2+ dependent 1K |
| Gene type | protein-coding |
| Also known as | BDP, MSUDMV, PP2Ckappa, PP2Cm, PTMP, UG0882E07 |
| Summary | This gene encodes a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. The encoded protein, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPM1K-210 | ENST00000608933.6 | 6309 | 372aa | Protein coding | CCDS3629 | Q8N3J5-1 | NM_152542.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PPM1K-209 | ENST00000514204.1 | 2962 | 182aa | Protein coding | - | V9GYZ5 | - | TSL:1, GENCODE basic, |
| PPM1K-201 | ENST00000295908.11 | 1940 | 327aa | Protein coding | - | A0A0A0MQZ4 | - | TSL:5, GENCODE basic, |
| PPM1K-204 | ENST00000508256.5 | 923 | 153aa | Protein coding | - | V9GY69 | - | TSL:2, GENCODE basic, |
| PPM1K-206 | ENST00000510548.6 | 572 | 21aa | Protein coding | - | V9GYI6 | - | CDS 3' incomplete, TSL:4, |
| PPM1K-202 | ENST00000505022.1 | 566 | 130aa | Protein coding | - | V9GYS8 | - | CDS 3' incomplete, TSL:3, |
| PPM1K-205 | ENST00000509340.1 | 563 | 5aa | Protein coding | - | - | - | CDS 3' incomplete, TSL:3, |
| PPM1K-203 | ENST00000506423.1 | 1091 | No protein | Processed transcript | - | - | - | TSL:1, |
| PPM1K-208 | ENST00000513546.3 | 4777 | No protein | Retained intron | - | - | - | TSL:NA, |
| PPM1K-207 | ENST00000511506.1 | 582 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPM1K |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPM1K had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: