CRISPR-U™ Gene Knockout Cell Line Strategy

PPP4R2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PPP4R2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPP4R2
Gene id 151987
Organism Homo sapiens
Official full symbol protein phosphatase 4 regulatory subunit 2
Gene type protein-coding
Also known as PP4R2
Summary The protein encoded by this gene is a regulatory subunit of the serine/threonine-protein phosphatase 4 complex. In addition to being required for efficient DNA double strand break repair, this complex plays a role in organization of microtubules at centrosomes and processing of spliceosomal snRNPs. Several transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 3
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPP4R2-201 ENST00000356692.10 4957 417aa Protein coding CCDS2917 Q9NY27-1 NM_174907.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PPP4R2-207 ENST00000495566.1 559 69aa Protein coding CCDS82805 C9IZF4 - TSL:3, GENCODE basic,
PPP4R2-206 ENST00000488810.5 877 226aa Protein coding - C9IZ04 - CDS 3' incomplete, TSL:5,
PPP4R2-202 ENST00000460360.1 572 143aa Protein coding - H7C559 - CDS 5' incomplete, TSL:4,
PPP4R2-204 ENST00000476505.2 323 62aa Protein coding - F8WDK3 - CDS 3' incomplete, TSL:5,
PPP4R2-205 ENST00000482242.5 771 116aa Nonsense mediated decay - F8WCA1 - TSL:3,
PPP4R2-203 ENST00000470976.1 1033 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPP4R2
This KO Strategy loading
Red Cotton™ Notes Gene PPP4R2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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