CRISPR-U™ Gene Knockout Cell Line
Strategy
PPP4R2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPP4R2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPP4R2 |
| Gene id | 151987 |
| Organism | Homo sapiens |
| Official full symbol | protein phosphatase 4 regulatory subunit 2 |
| Gene type | protein-coding |
| Also known as | PP4R2 |
| Summary | The protein encoded by this gene is a regulatory subunit of the serine/threonine-protein phosphatase 4 complex. In addition to being required for efficient DNA double strand break repair, this complex plays a role in organization of microtubules at centrosomes and processing of spliceosomal snRNPs. Several transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPP4R2-201 | ENST00000356692.10 | 4957 | 417aa | Protein coding | CCDS2917 | Q9NY27-1 | NM_174907.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PPP4R2-207 | ENST00000495566.1 | 559 | 69aa | Protein coding | CCDS82805 | C9IZF4 | - | TSL:3, GENCODE basic, |
| PPP4R2-206 | ENST00000488810.5 | 877 | 226aa | Protein coding | - | C9IZ04 | - | CDS 3' incomplete, TSL:5, |
| PPP4R2-202 | ENST00000460360.1 | 572 | 143aa | Protein coding | - | H7C559 | - | CDS 5' incomplete, TSL:4, |
| PPP4R2-204 | ENST00000476505.2 | 323 | 62aa | Protein coding | - | F8WDK3 | - | CDS 3' incomplete, TSL:5, |
| PPP4R2-205 | ENST00000482242.5 | 771 | 116aa | Nonsense mediated decay | - | F8WCA1 | - | TSL:3, |
| PPP4R2-203 | ENST00000470976.1 | 1033 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPP4R2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPP4R2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: