CRISPR-U™ Gene Knockout Cell Line
Strategy
DTX3L Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DTX3L Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DTX3L |
| Gene id | 151636 |
| Organism | Homo sapiens |
| Official full symbol | deltex E3 ubiquitin ligase 3L |
| Gene type | protein-coding |
| Also known as | BBAP, RNF143 |
| Summary | DTX3L functions as an E3 ubiquitin ligase (Takeyama et al., 2003 ). |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DTX3L-201 | ENST00000296161.9 | 5768 | 740aa | Protein coding | CCDS3015 | Q8TDB6-1 | NM_138287.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DTX3L-202 | ENST00000383661.3 | 687 | 228aa | Protein coding | - | Q8TDB6-2 | - | TSL:1, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DTX3L |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DTX3L had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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