CRISPR-U™ Gene Knockout Cell Line Strategy

DTX3L Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DTX3L Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DTX3L
Gene id 151636
Organism Homo sapiens
Official full symbol deltex E3 ubiquitin ligase 3L
Gene type protein-coding
Also known as BBAP, RNF143
Summary DTX3L functions as an E3 ubiquitin ligase (Takeyama et al., 2003 ).
Genomic regions Chromosome 3
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DTX3L-201 ENST00000296161.9 5768 740aa Protein coding CCDS3015 Q8TDB6-1 NM_138287.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DTX3L-202 ENST00000383661.3 687 228aa Protein coding - Q8TDB6-2 - TSL:1, GENCODE basic,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DTX3L
This KO Strategy loading
Red Cotton™ Notes Gene DTX3L had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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