CRISPR-U™ Gene Knockout Cell Line
Strategy
CTSL Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CTSL Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CTSL |
| Gene id | 1514 |
| Organism | Homo sapiens |
| Official full symbol | cathepsin L |
| Gene type | protein-coding |
| Also known as | CATL, CTSL1, MEP |
| Summary | The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Additionally, this protein may be necessary for entry of SARS-CoV-2 (COVID-19 virus) into the cell. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 20 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CTSL-223 | ENST00000677761.1 | 2397 | 333aa | Protein coding | CCDS6675 | A0A024R276 | - | GENCODE basic, APPRIS P1, |
| CTSL-208 | ENST00000676480.1 | 2392 | 333aa | Protein coding | CCDS6675 | A0A024R276 | - | GENCODE basic, APPRIS P1, |
| CTSL-241 | ENST00000679149.1 | 2129 | 333aa | Protein coding | CCDS6675 | A0A024R276 | - | GENCODE basic, APPRIS P1, |
| CTSL-242 | ENST00000679157.1 | 1981 | 333aa | Protein coding | CCDS6675 | A0A024R276 | - | GENCODE basic, APPRIS P1, |
| CTSL-209 | ENST00000676531.1 | 1813 | 333aa | Protein coding | CCDS6675 | A0A024R276 | - | GENCODE basic, APPRIS P1, |
| CTSL-224 | ENST00000677821.1 | 1719 | 333aa | Protein coding | CCDS6675 | A0A024R276 | - | GENCODE basic, APPRIS P1, |
| CTSL-203 | ENST00000343150.10 | 1654 | 333aa | Protein coding | CCDS6675 | A0A024R276 P07711 | NM_001912.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CTSL-217 | ENST00000677262.1 | 1613 | 333aa | Protein coding | CCDS6675 | A0A024R276 | - | GENCODE basic, APPRIS P1, |
| CTSL-212 | ENST00000676881.1 | 1593 | 333aa | Protein coding | CCDS6675 | A0A024R276 | - | GENCODE basic, APPRIS P1, |
| CTSL-201 | ENST00000340342.11 | 1536 | 333aa | Protein coding | CCDS6675 | A0A024R276 P07711 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CTSL-214 | ENST00000676946.1 | 1420 | 333aa | Protein coding | CCDS6675 | A0A024R276 | - | GENCODE basic, APPRIS P1, |
| CTSL-230 | ENST00000678442.1 | 1394 | 333aa | Protein coding | CCDS6675 | A0A024R276 | - | GENCODE basic, APPRIS P1, |
| CTSL-231 | ENST00000678596.1 | 1963 | 341aa | Protein coding | - | - | - | GENCODE basic, |
| CTSL-233 | ENST00000678649.1 | 1897 | 341aa | Protein coding | - | - | - | GENCODE basic, |
| CTSL-225 | ENST00000677864.1 | 1373 | 278aa | Protein coding | - | - | - | GENCODE basic, |
| CTSL-211 | ENST00000676769.1 | 1329 | 278aa | Protein coding | - | - | - | GENCODE basic, |
| CTSL-202 | ENST00000342020.6 | 1315 | 272aa | Protein coding | - | Q5T8F0 | - | TSL:2, GENCODE basic, |
| CTSL-232 | ENST00000678599.1 | 1196 | 258aa | Protein coding | - | - | - | GENCODE basic, |
| CTSL-216 | ENST00000677019.1 | 1100 | 278aa | Protein coding | - | - | - | GENCODE basic, |
| CTSL-239 | ENST00000679030.1 | 1010 | 209aa | Protein coding | - | - | - | GENCODE basic, |
| CTSL-222 | ENST00000677638.1 | 2682 | 64aa | Nonsense mediated decay | - | - | - | - |
| CTSL-238 | ENST00000679028.1 | 2037 | 64aa | Nonsense mediated decay | - | - | - | - |
| CTSL-237 | ENST00000679025.1 | 1650 | 225aa | Nonsense mediated decay | - | - | - | - |
| CTSL-229 | ENST00000678367.1 | 1578 | 64aa | Nonsense mediated decay | - | - | - | - |
| CTSL-227 | ENST00000677955.1 | 1555 | 64aa | Nonsense mediated decay | - | - | - | - |
| CTSL-228 | ENST00000678259.1 | 1369 | 83aa | Nonsense mediated decay | - | - | - | - |
| CTSL-220 | ENST00000677523.1 | 1221 | 233aa | Nonsense mediated decay | - | - | - | - |
| CTSL-215 | ENST00000676961.1 | 4588 | No protein | Retained intron | - | - | - | - |
| CTSL-218 | ENST00000677345.1 | 3930 | No protein | Retained intron | - | - | - | - |
| CTSL-243 | ENST00000679252.1 | 3848 | No protein | Retained intron | - | - | - | - |
| CTSL-213 | ENST00000676909.1 | 3663 | No protein | Retained intron | - | - | - | - |
| CTSL-205 | ENST00000482054.2 | 2786 | No protein | Retained intron | - | - | - | TSL:2, |
| CTSL-210 | ENST00000676576.1 | 2765 | No protein | Retained intron | - | - | - | - |
| CTSL-236 | ENST00000678965.1 | 2678 | No protein | Retained intron | - | - | - | - |
| CTSL-207 | ENST00000676466.1 | 2650 | No protein | Retained intron | - | - | - | - |
| CTSL-235 | ENST00000678752.1 | 2585 | No protein | Retained intron | - | - | - | - |
| CTSL-240 | ENST00000679141.1 | 2517 | No protein | Retained intron | - | - | - | - |
| CTSL-226 | ENST00000677935.1 | 2204 | No protein | Retained intron | - | - | - | - |
| CTSL-219 | ENST00000677349.1 | 2092 | No protein | Retained intron | - | - | - | - |
| CTSL-221 | ENST00000677615.1 | 2058 | No protein | Retained intron | - | - | - | - |
| CTSL-204 | ENST00000375894.10 | 1541 | No protein | Retained intron | - | - | - | TSL:3, |
| CTSL-206 | ENST00000495822.2 | 1345 | No protein | Retained intron | - | - | - | TSL:1, |
| CTSL-234 | ENST00000678654.1 | 1345 | No protein | Retained intron | - | - | - | - |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CTSL |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CTSL had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
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$599
Single gRNA lentivirus
Work flow
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