CRISPR-U™ Gene Knockout Cell Line
Strategy
CTSH Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CTSH Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CTSH |
| Gene id | 1512 |
| Organism | Homo sapiens |
| Official full symbol | cathepsin H |
| Gene type | protein-coding |
| Also known as | ACC-4, ACC-5, ACC4, ACC5, CPSB |
| Summary | The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CTSH-201 | ENST00000220166.10 | 2145 | 335aa | Protein coding | CCDS10308 | P09668 | NM_004390.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CTSH-233 | ENST00000677011.1 | 2169 | 259aa | Protein coding | - | - | - | GENCODE basic, |
| CTSH-248 | ENST00000677936.1 | 2127 | 311aa | Protein coding | - | - | - | GENCODE basic, |
| CTSH-245 | ENST00000677810.1 | 2019 | 293aa | Protein coding | - | - | - | GENCODE basic, |
| CTSH-243 | ENST00000677534.1 | 1760 | 446aa | Protein coding | - | - | - | GENCODE basic, |
| CTSH-257 | ENST00000678644.1 | 1601 | 259aa | Protein coding | - | - | - | GENCODE basic, |
| CTSH-239 | ENST00000677316.1 | 1540 | 297aa | Protein coding | - | - | - | GENCODE basic, |
| CTSH-232 | ENST00000676880.1 | 1513 | 367aa | Protein coding | - | - | - | GENCODE basic, |
| CTSH-207 | ENST00000528741.6 | 1341 | 297aa | Protein coding | - | E9PKT6 | - | TSL:5, GENCODE basic, |
| CTSH-236 | ENST00000677207.1 | 1339 | 251aa | Protein coding | - | - | - | GENCODE basic, |
| CTSH-218 | ENST00000615999.5 | 1286 | 358aa | Protein coding | - | A0A087X0D5 | - | TSL:1, GENCODE basic, |
| CTSH-244 | ENST00000677789.1 | 1198 | 306aa | Protein coding | - | - | - | GENCODE basic, |
| CTSH-230 | ENST00000676850.1 | 1136 | 345aa | Non stop decay | - | - | - | - |
| CTSH-268 | ENST00000679211.1 | 6954 | 41aa | Nonsense mediated decay | - | - | - | - |
| CTSH-221 | ENST00000676510.1 | 4540 | 341aa | Nonsense mediated decay | - | - | - | - |
| CTSH-259 | ENST00000678799.1 | 4029 | 41aa | Nonsense mediated decay | - | - | - | - |
| CTSH-224 | ENST00000676639.1 | 3392 | 341aa | Nonsense mediated decay | - | - | - | - |
| CTSH-240 | ENST00000677320.1 | 2511 | 41aa | Nonsense mediated decay | - | - | - | - |
| CTSH-264 | ENST00000679017.1 | 2434 | 260aa | Nonsense mediated decay | - | - | - | - |
| CTSH-269 | ENST00000679334.1 | 2194 | 240aa | Nonsense mediated decay | - | - | - | - |
| CTSH-228 | ENST00000676763.1 | 2173 | 85aa | Nonsense mediated decay | - | - | - | - |
| CTSH-213 | ENST00000533777.5 | 2101 | 62aa | Nonsense mediated decay | - | A0A0B4J217 | - | TSL:1, |
| CTSH-258 | ENST00000678727.1 | 2063 | 200aa | Nonsense mediated decay | - | - | - | - |
| CTSH-265 | ENST00000679047.1 | 1987 | 170aa | Nonsense mediated decay | - | - | - | - |
| CTSH-247 | ENST00000677921.1 | 1942 | 41aa | Nonsense mediated decay | - | - | - | - |
| CTSH-262 | ENST00000678886.1 | 1881 | 41aa | Nonsense mediated decay | - | - | - | - |
| CTSH-253 | ENST00000678283.1 | 1717 | 238aa | Nonsense mediated decay | - | - | - | - |
| CTSH-223 | ENST00000676596.1 | 1611 | 238aa | Nonsense mediated decay | - | - | - | - |
| CTSH-249 | ENST00000678031.1 | 1607 | 170aa | Nonsense mediated decay | - | - | - | - |
| CTSH-219 | ENST00000649928.2 | 1576 | 169aa | Nonsense mediated decay | - | A0A3B3IRM7 | - | - |
| CTSH-246 | ENST00000677874.1 | 1564 | 38aa | Nonsense mediated decay | - | - | - | - |
| CTSH-252 | ENST00000678281.1 | 1560 | 138aa | Nonsense mediated decay | - | - | - | - |
| CTSH-267 | ENST00000679172.1 | 1524 | 62aa | Nonsense mediated decay | - | - | - | - |
| CTSH-256 | ENST00000678487.1 | 1506 | 43aa | Nonsense mediated decay | - | - | - | - |
| CTSH-229 | ENST00000676808.1 | 1503 | 48aa | Nonsense mediated decay | - | - | - | - |
| CTSH-255 | ENST00000678415.1 | 1487 | 49aa | Nonsense mediated decay | - | - | - | - |
| CTSH-205 | ENST00000528191.6 | 1458 | 86aa | Nonsense mediated decay | - | - | - | TSL:4, |
| CTSH-202 | ENST00000525807.6 | 1386 | 38aa | Nonsense mediated decay | - | E9PN60 | - | TSL:5, |
| CTSH-210 | ENST00000529861.6 | 1185 | 41aa | Nonsense mediated decay | - | E9PN84 | - | TSL:3, |
| CTSH-241 | ENST00000677367.1 | 1889 | No protein | Processed transcript | - | - | - | - |
| CTSH-217 | ENST00000534533.6 | 1526 | No protein | Processed transcript | - | - | - | TSL:3, |
| CTSH-209 | ENST00000529612.6 | 7215 | No protein | Retained intron | - | - | - | TSL:4, |
| CTSH-261 | ENST00000678841.1 | 7061 | No protein | Retained intron | - | - | - | - |
| CTSH-263 | ENST00000678940.1 | 6655 | No protein | Retained intron | - | - | - | - |
| CTSH-203 | ENST00000527138.6 | 5163 | No protein | Retained intron | - | - | - | TSL:4, |
| CTSH-211 | ENST00000530010.6 | 4798 | No protein | Retained intron | - | - | - | TSL:2, |
| CTSH-251 | ENST00000678114.1 | 4445 | No protein | Retained intron | - | - | - | - |
| CTSH-238 | ENST00000677254.1 | 4424 | No protein | Retained intron | - | - | - | - |
| CTSH-254 | ENST00000678397.1 | 4142 | No protein | Retained intron | - | - | - | - |
| CTSH-220 | ENST00000676486.1 | 4132 | No protein | Retained intron | - | - | - | - |
| CTSH-234 | ENST00000677049.1 | 4076 | No protein | Retained intron | - | - | - | - |
| CTSH-237 | ENST00000677238.1 | 3940 | No protein | Retained intron | - | - | - | - |
| CTSH-204 | ENST00000527715.6 | 3693 | No protein | Retained intron | - | - | - | TSL:1, |
| CTSH-260 | ENST00000678817.1 | 3413 | No protein | Retained intron | - | - | - | - |
| CTSH-225 | ENST00000676668.1 | 3218 | No protein | Retained intron | - | - | - | - |
| CTSH-226 | ENST00000676671.1 | 2445 | No protein | Retained intron | - | - | - | - |
| CTSH-215 | ENST00000534237.6 | 2100 | No protein | Retained intron | - | - | - | TSL:3, |
| CTSH-231 | ENST00000676865.1 | 1903 | No protein | Retained intron | - | - | - | - |
| CTSH-222 | ENST00000676588.1 | 1850 | No protein | Retained intron | - | - | - | - |
| CTSH-266 | ENST00000679125.1 | 1797 | No protein | Retained intron | - | - | - | - |
| CTSH-242 | ENST00000677448.1 | 1783 | No protein | Retained intron | - | - | - | - |
| CTSH-235 | ENST00000677102.1 | 1686 | No protein | Retained intron | - | - | - | - |
| CTSH-250 | ENST00000678033.1 | 1621 | No protein | Retained intron | - | - | - | - |
| CTSH-216 | ENST00000534268.6 | 1444 | No protein | Retained intron | - | - | - | TSL:5, |
| CTSH-227 | ENST00000676738.1 | 1157 | No protein | Retained intron | - | - | - | - |
| CTSH-214 | ENST00000534038.6 | 871 | No protein | Retained intron | - | - | - | TSL:4, |
| CTSH-208 | ENST00000529263.7 | 865 | No protein | Retained intron | - | - | - | TSL:5, |
| CTSH-206 | ENST00000528436.2 | 572 | No protein | Retained intron | - | - | - | TSL:3, |
| CTSH-212 | ENST00000530929.5 | 479 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CTSH |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CTSH had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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