CRISPR-U™ Gene Knockout Cell Line
Strategy
PYHIN1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PYHIN1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PYHIN1 |
| Gene id | 149628 |
| Organism | Homo sapiens |
| Official full symbol | pyrin and HIN domain family member 1 |
| Gene type | protein-coding |
| Also known as | IFIX |
| Summary | The protein encoded by this gene belongs to the HIN-200 family of interferon-inducible proteins that share a 200-amino acid signature motif at their C-termini. HIN200 proteins are primarily nuclear and are involved in transcriptional regulation of genes important for cell cycle control, differentiation, and apoptosis. Downregulation of this gene is associated with breast cancer. This protein acts as a tumor suppressor by promoting ubiquitination and subsequent degradation of MDM2, which leads to stabilization of p53/TP53. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PYHIN1-203 | ENST00000368140.6 | 2088 | 492aa | Protein coding | CCDS1178 | Q6K0P9-1 | NM_152501.5 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| PYHIN1-202 | ENST00000368138.7 | 1723 | 483aa | Protein coding | CCDS1179 | Q6K0P9-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PYHIN1-205 | ENST00000392254.6 | 1624 | 461aa | Protein coding | CCDS30907 | Q6K0P9-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PYHIN1-204 | ENST00000392252.7 | 1597 | 452aa | Protein coding | CCDS30908 | Q6K0P9-4 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PYHIN1-201 | ENST00000368135.4 | 1662 | 245aa | Protein coding | - | Q6K0P9-5 | - | TSL:1, GENCODE basic, |
| PYHIN1-206 | ENST00000458222.5 | 771 | 150aa | Protein coding | - | X6RHR0 | - | CDS 3' incomplete, TSL:5, |
| PYHIN1-207 | ENST00000485134.1 | 1129 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PYHIN1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PYHIN1 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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