CRISPR-U™ Gene Knockout Cell Line Strategy

PYHIN1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PYHIN1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PYHIN1
Gene id 149628
Organism Homo sapiens
Official full symbol pyrin and HIN domain family member 1
Gene type protein-coding
Also known as IFIX
Summary The protein encoded by this gene belongs to the HIN-200 family of interferon-inducible proteins that share a 200-amino acid signature motif at their C-termini. HIN200 proteins are primarily nuclear and are involved in transcriptional regulation of genes important for cell cycle control, differentiation, and apoptosis. Downregulation of this gene is associated with breast cancer. This protein acts as a tumor suppressor by promoting ubiquitination and subsequent degradation of MDM2, which leads to stabilization of p53/TP53. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PYHIN1-203 ENST00000368140.6 2088 492aa Protein coding CCDS1178 Q6K0P9-1 NM_152501.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PYHIN1-202 ENST00000368138.7 1723 483aa Protein coding CCDS1179 Q6K0P9-2 - TSL:1, GENCODE basic, APPRIS ALT2,
PYHIN1-205 ENST00000392254.6 1624 461aa Protein coding CCDS30907 Q6K0P9-3 - TSL:1, GENCODE basic, APPRIS ALT2,
PYHIN1-204 ENST00000392252.7 1597 452aa Protein coding CCDS30908 Q6K0P9-4 - TSL:1, GENCODE basic, APPRIS ALT2,
PYHIN1-201 ENST00000368135.4 1662 245aa Protein coding - Q6K0P9-5 - TSL:1, GENCODE basic,
PYHIN1-206 ENST00000458222.5 771 150aa Protein coding - X6RHR0 - CDS 3' incomplete, TSL:5,
PYHIN1-207 ENST00000485134.1 1129 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PYHIN1
This KO Strategy loading
Red Cotton™ Notes Gene PYHIN1 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×