CRISPR-U™ Gene Knockout Cell Line Strategy

CTLA4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CTLA4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CTLA4
Gene id 1493
Organism Homo sapiens
Official full symbol cytotoxic T-lymphocyte associated protein 4
Gene type protein-coding
Also known as ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12
Summary This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Genomic regions Chromosome 2
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CTLA4-205 ENST00000648405.2 1997 223aa Protein coding CCDS2362 P16410-1 NM_005214.5 GENCODE basic, APPRIS P1, MANE Select v0.92,
CTLA4-201 ENST00000295854.10 525 174aa Protein coding CCDS42803 P16410-5 - TSL:5, GENCODE basic,
CTLA4-203 ENST00000472206.1 277 79aa Protein coding - P16410-4 - TSL:1, GENCODE basic,
CTLA4-204 ENST00000487393.1 214 58aa Protein coding - P16410-3 - TSL:1, GENCODE basic,
CTLA4-202 ENST00000427473.3 874 No protein Processed transcript - - - TSL:5,
CTLA4-206 ENST00000650075.1 740 No protein Processed transcript - - - -
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CTLA4
This KO Strategy loading
Red Cotton™ Notes Gene CTLA4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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