CRISPR-U™ Gene Knockout Cell Line
Strategy
CTLA4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CTLA4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CTLA4 |
| Gene id | 1493 |
| Organism | Homo sapiens |
| Official full symbol | cytotoxic T-lymphocyte associated protein 4 |
| Gene type | protein-coding |
| Also known as | ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12 |
| Summary | This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CTLA4-205 | ENST00000648405.2 | 1997 | 223aa | Protein coding | CCDS2362 | P16410-1 | NM_005214.5 | GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CTLA4-201 | ENST00000295854.10 | 525 | 174aa | Protein coding | CCDS42803 | P16410-5 | - | TSL:5, GENCODE basic, |
| CTLA4-203 | ENST00000472206.1 | 277 | 79aa | Protein coding | - | P16410-4 | - | TSL:1, GENCODE basic, |
| CTLA4-204 | ENST00000487393.1 | 214 | 58aa | Protein coding | - | P16410-3 | - | TSL:1, GENCODE basic, |
| CTLA4-202 | ENST00000427473.3 | 874 | No protein | Processed transcript | - | - | - | TSL:5, |
| CTLA4-206 | ENST00000650075.1 | 740 | No protein | Processed transcript | - | - | - | - |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CTLA4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CTLA4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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