CRISPR-U™ Gene Knockout Cell Line
Strategy
CREB3L4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CREB3L4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CREB3L4 |
| Gene id | 148327 |
| Organism | Homo sapiens |
| Official full symbol | cAMP responsive element binding protein 3 like 4 |
| Gene type | protein-coding |
| Also known as | AIBZIP, ATCE1, CREB3, CREB4, JAL, hJAL |
| Summary | This gene encodes a CREB (cAMP responsive element binding) protein with a transmembrane domain which localizes it to the ER membrane. The encoded protein is a transcriptional activator which contains a dimerization domain, and this protein may function in a number of processing pathways including protein processing. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CREB3L4-201 | ENST00000271889.8 | 1750 | 395aa | Protein coding | CCDS1056 | Q8TEY5-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| CREB3L4-205 | ENST00000368607.8 | 1749 | 395aa | Protein coding | CCDS1056 | Q8TEY5-1 | NM_001255978.2 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| CREB3L4-204 | ENST00000368603.5 | 1557 | 395aa | Protein coding | CCDS1056 | Q8TEY5-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| CREB3L4-202 | ENST00000368600.7 | 1496 | 375aa | Protein coding | CCDS58029 | Q8TEY5-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| CREB3L4-207 | ENST00000449724.5 | 982 | 228aa | Protein coding | - | Q5T4L1 | - | CDS 3' incomplete, TSL:5, |
| CREB3L4-203 | ENST00000368601.5 | 950 | 217aa | Protein coding | - | Q5T4K9 | - | TSL:2, GENCODE basic, |
| CREB3L4-206 | ENST00000431292.1 | 602 | 182aa | Protein coding | - | Q5T4L2 | - | CDS 3' incomplete, TSL:5, |
| CREB3L4-209 | ENST00000468845.1 | 906 | No protein | Processed transcript | - | - | - | TSL:3, |
| CREB3L4-210 | ENST00000473340.1 | 880 | No protein | Processed transcript | - | - | - | TSL:2, |
| CREB3L4-208 | ENST00000461688.5 | 870 | No protein | Processed transcript | - | - | - | TSL:5, |
| CREB3L4-213 | ENST00000492729.1 | 835 | No protein | Processed transcript | - | - | - | TSL:5, |
| CREB3L4-212 | ENST00000479010.1 | 612 | No protein | Processed transcript | - | - | - | TSL:2, |
| CREB3L4-211 | ENST00000477617.5 | 568 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CREB3L4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CREB3L4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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