CRISPR-U™ Gene Knockout Cell Line Strategy

CSTB Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CSTB Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CSTB
Gene id 1476
Organism Homo sapiens
Official full symbol cystatin B
Gene type protein-coding
Also known as CPI-B, CST6, EPM1, EPM1A, PME, STFB, ULD
Summary The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.
Genomic regions Chromosome 21
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CSTB-201 ENST00000291568.7 588 98aa Protein coding CCDS13701 P04080 Q76LA1 NM_000100.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CSTB-204 ENST00000640406.1 2354 83aa Protein coding - A0A1W2PS52 - TSL:2, GENCODE basic,
CSTB-203 ENST00000639959.1 1855 54aa Protein coding - A0A1W2PQG6 - CDS 5' incomplete, TSL:5,
CSTB-205 ENST00000675996.1 1258 No protein Processed transcript - - - -
CSTB-202 ENST00000480147.3 3744 No protein Retained intron - - - TSL:NA,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CSTB
This KO Strategy loading
Red Cotton™ Notes Gene CSTB had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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