CRISPR-U™ Gene Knockout Cell Line
Strategy
CSTB Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CSTB Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CSTB |
| Gene id | 1476 |
| Organism | Homo sapiens |
| Official full symbol | cystatin B |
| Gene type | protein-coding |
| Also known as | CPI-B, CST6, EPM1, EPM1A, PME, STFB, ULD |
| Summary | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies. |
| Genomic regions | Chromosome 21 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CSTB-201 | ENST00000291568.7 | 588 | 98aa | Protein coding | CCDS13701 | P04080 Q76LA1 | NM_000100.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CSTB-204 | ENST00000640406.1 | 2354 | 83aa | Protein coding | - | A0A1W2PS52 | - | TSL:2, GENCODE basic, |
| CSTB-203 | ENST00000639959.1 | 1855 | 54aa | Protein coding | - | A0A1W2PQG6 | - | CDS 5' incomplete, TSL:5, |
| CSTB-205 | ENST00000675996.1 | 1258 | No protein | Processed transcript | - | - | - | - |
| CSTB-202 | ENST00000480147.3 | 3744 | No protein | Retained intron | - | - | - | TSL:NA, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CSTB |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CSTB had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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