CRISPR-U™ Gene Knockout Cell Line
Strategy
CSRP1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CSRP1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CSRP1 |
| Gene id | 1465 |
| Organism | Homo sapiens |
| Official full symbol | cysteine and glycine rich protein 1 |
| Gene type | protein-coding |
| Also known as | CRP, CRP1, CSRP, CYRP, D1S181E, HEL-141, HEL-S-286 |
| Summary | This gene encodes a member of the cysteine-rich protein (CSRP) family. This gene family includes a group of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this gene product occurs in proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Alternatively spliced transcript variants have been described. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CSRP1-202 | ENST00000367306.5 | 2129 | 193aa | Protein coding | CCDS1413 | A0A384P5K2 P21291 | - | TSL:5, GENCODE basic, APPRIS P1, |
| CSRP1-201 | ENST00000340006.7 | 1820 | 193aa | Protein coding | CCDS1413 | A0A384P5K2 P21291 | NM_004078.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CSRP1-216 | ENST00000532460.5 | 1469 | 193aa | Protein coding | CCDS1413 | A0A384P5K2 P21291 | - | TSL:2, GENCODE basic, APPRIS P1, |
| CSRP1-219 | ENST00000533432.5 | 1149 | 193aa | Protein coding | CCDS1413 | A0A384P5K2 P21291 | - | TSL:2, GENCODE basic, APPRIS P1, |
| CSRP1-214 | ENST00000531916.5 | 890 | 153aa | Protein coding | - | E9PS42 | - | TSL:5, GENCODE basic, |
| CSRP1-206 | ENST00000524951.5 | 582 | 153aa | Protein coding | - | E9PND2 | - | CDS 3' incomplete, TSL:2, |
| CSRP1-207 | ENST00000526256.5 | 582 | 17aa | Protein coding | - | A0A087X134 | - | TSL:5, GENCODE basic, |
| CSRP1-209 | ENST00000526723.5 | 574 | 160aa | Protein coding | - | E9PP21 | - | TSL:3, GENCODE basic, |
| CSRP1-203 | ENST00000458271.6 | 1324 | No protein | Processed transcript | - | - | - | TSL:2, |
| CSRP1-213 | ENST00000530120.5 | 568 | No protein | Processed transcript | - | - | - | TSL:4, |
| CSRP1-210 | ENST00000527573.5 | 567 | No protein | Processed transcript | - | - | - | TSL:2, |
| CSRP1-212 | ENST00000529975.5 | 548 | No protein | Processed transcript | - | - | - | TSL:4, |
| CSRP1-208 | ENST00000526317.5 | 374 | No protein | Processed transcript | - | - | - | TSL:3, |
| CSRP1-218 | ENST00000533402.5 | 8475 | No protein | Retained intron | - | - | - | TSL:1, |
| CSRP1-215 | ENST00000532313.5 | 5239 | No protein | Retained intron | - | - | - | TSL:1, |
| CSRP1-211 | ENST00000527662.5 | 4208 | No protein | Retained intron | - | - | - | TSL:2, |
| CSRP1-204 | ENST00000471596.1 | 758 | No protein | Retained intron | - | - | - | TSL:2, |
| CSRP1-205 | ENST00000481851.1 | 741 | No protein | Retained intron | - | - | - | TSL:2, |
| CSRP1-217 | ENST00000533188.1 | 581 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CSRP1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CSRP1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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