CRISPR-U™ Gene Knockout Cell Line
Strategy
NRG4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NRG4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NRG4 |
| Gene id | 145957 |
| Organism | Homo sapiens |
| Official full symbol | neuregulin 4 |
| Gene type | protein-coding |
| Also known as | HRG4 |
| Summary | The neuregulins, including NRG4, activate type-1 growth factor receptors (see EGFR; MIM 131550) to initiating cell-to-cell signaling through tyrosine phosphorylation (Harari et al., 1999 ). |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NRG4-201 | ENST00000394907.8 | 3212 | 115aa | Protein coding | CCDS10288 | Q8WWG1 | NM_138573.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NRG4-208 | ENST00000563910.5 | 799 | 110aa | Protein coding | - | H3BP63 | - | CDS 3' incomplete, TSL:5, |
| NRG4-210 | ENST00000566417.5 | 581 | 115aa | Nonsense mediated decay | CCDS10288 | Q8WWG1 | - | TSL:3, |
| NRG4-202 | ENST00000461391.5 | 3406 | 35aa | Nonsense mediated decay | - | E9PJI7 | - | TSL:1, |
| NRG4-203 | ENST00000472094.5 | 2368 | 36aa | Nonsense mediated decay | - | Q7Z663 | - | TSL:2, |
| NRG4-209 | ENST00000565661.5 | 1453 | 35aa | Nonsense mediated decay | - | E9PJI7 | - | TSL:2, |
| NRG4-205 | ENST00000562114.5 | 1036 | 36aa | Nonsense mediated decay | - | Q7Z663 | - | TSL:2, |
| NRG4-214 | ENST00000568073.5 | 1007 | 36aa | Nonsense mediated decay | - | Q7Z663 | - | TSL:1, |
| NRG4-211 | ENST00000567126.5 | 716 | 36aa | Nonsense mediated decay | - | Q7Z663 | - | TSL:3, |
| NRG4-206 | ENST00000563204.5 | 610 | 90aa | Nonsense mediated decay | - | Q0P6N6 | - | TSL:5, |
| NRG4-213 | ENST00000567936.5 | 559 | 36aa | Nonsense mediated decay | - | Q7Z663 | - | TSL:5, |
| NRG4-204 | ENST00000498750.5 | 529 | 36aa | Nonsense mediated decay | - | Q7Z663 | - | TSL:4, |
| NRG4-207 | ENST00000563582.1 | 558 | No protein | Processed transcript | - | - | - | TSL:4, |
| NRG4-216 | ENST00000569343.5 | 513 | No protein | Processed transcript | - | - | - | TSL:5, |
| NRG4-212 | ENST00000567467.5 | 477 | No protein | Processed transcript | - | - | - | TSL:4, |
| NRG4-215 | ENST00000568203.1 | 166 | No protein | Processed transcript | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NRG4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NRG4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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