CRISPR-U™ Gene Knockout Cell Line Strategy

NRG4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NRG4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NRG4
Gene id 145957
Organism Homo sapiens
Official full symbol neuregulin 4
Gene type protein-coding
Also known as HRG4
Summary The neuregulins, including NRG4, activate type-1 growth factor receptors (see EGFR; MIM 131550) to initiating cell-to-cell signaling through tyrosine phosphorylation (Harari et al., 1999 ).
Genomic regions Chromosome 15
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NRG4-201 ENST00000394907.8 3212 115aa Protein coding CCDS10288 Q8WWG1 NM_138573.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NRG4-208 ENST00000563910.5 799 110aa Protein coding - H3BP63 - CDS 3' incomplete, TSL:5,
NRG4-210 ENST00000566417.5 581 115aa Nonsense mediated decay CCDS10288 Q8WWG1 - TSL:3,
NRG4-202 ENST00000461391.5 3406 35aa Nonsense mediated decay - E9PJI7 - TSL:1,
NRG4-203 ENST00000472094.5 2368 36aa Nonsense mediated decay - Q7Z663 - TSL:2,
NRG4-209 ENST00000565661.5 1453 35aa Nonsense mediated decay - E9PJI7 - TSL:2,
NRG4-205 ENST00000562114.5 1036 36aa Nonsense mediated decay - Q7Z663 - TSL:2,
NRG4-214 ENST00000568073.5 1007 36aa Nonsense mediated decay - Q7Z663 - TSL:1,
NRG4-211 ENST00000567126.5 716 36aa Nonsense mediated decay - Q7Z663 - TSL:3,
NRG4-206 ENST00000563204.5 610 90aa Nonsense mediated decay - Q0P6N6 - TSL:5,
NRG4-213 ENST00000567936.5 559 36aa Nonsense mediated decay - Q7Z663 - TSL:5,
NRG4-204 ENST00000498750.5 529 36aa Nonsense mediated decay - Q7Z663 - TSL:4,
NRG4-207 ENST00000563582.1 558 No protein Processed transcript - - - TSL:4,
NRG4-216 ENST00000569343.5 513 No protein Processed transcript - - - TSL:5,
NRG4-212 ENST00000567467.5 477 No protein Processed transcript - - - TSL:4,
NRG4-215 ENST00000568203.1 166 No protein Processed transcript - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NRG4
This KO Strategy loading
Red Cotton™ Notes Gene NRG4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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