CRISPR-U™ Gene Knockout Cell Line Strategy

HAPLN3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HAPLN3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HAPLN3
Gene id 145864
Organism Homo sapiens
Official full symbol hyaluronan and proteoglycan link protein 3
Gene type protein-coding
Also known as EXLD1, HsT19883
Summary This gene belongs to the hyaluronan and proteoglycan binding link protein gene family. The protein encoded by this gene may function in hyaluronic acid binding and cell adhesion.
Genomic regions Chromosome 15
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HAPLN3-201 ENST00000359595.8 1892 360aa Protein coding CCDS10346 A0A024RC58 Q96S86 NM_178232.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
HAPLN3-204 ENST00000562889.5 1547 422aa Protein coding CCDS76790 H3BTH8 - TSL:5, GENCODE basic, APPRIS ALT2,
HAPLN3-203 ENST00000562281.1 801 226aa Protein coding - H3BT26 - CDS 3' incomplete, TSL:3,
HAPLN3-202 ENST00000558770.5 3071 186aa Nonsense mediated decay - H3BRY4 - TSL:2,
HAPLN3-205 ENST00000563808.1 807 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HAPLN3
This KO Strategy loading
Red Cotton™ Notes Gene HAPLN3 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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