CRISPR-U™ Gene Knockout Cell Line
Strategy
CSNK2A1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CSNK2A1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CSNK2A1 |
| Gene id | 1457 |
| Organism | Homo sapiens |
| Official full symbol | casein kinase 2 alpha 1 |
| Gene type | protein-coding |
| Also known as | CK2A1, CKII, Cka1, Cka2, OCNDS |
| Summary | Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 30 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CSNK2A1-201 | ENST00000217244.9 | 12984 | 391aa | Protein coding | CCDS13003 | P68400-1 | NM_177559.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CSNK2A1-243 | ENST00000646561.1 | 12912 | 391aa | Protein coding | CCDS13003 | P68400-1 | - | GENCODE basic, APPRIS P1, |
| CSNK2A1-240 | ENST00000646305.1 | 4459 | 391aa | Protein coding | CCDS13003 | P68400-1 | - | GENCODE basic, APPRIS P1, |
| CSNK2A1-202 | ENST00000349736.10 | 4047 | 255aa | Protein coding | CCDS13004 | P68400-2 | - | TSL:1, GENCODE basic, |
| CSNK2A1-217 | ENST00000643660.1 | 2822 | 391aa | Protein coding | CCDS13003 | P68400-1 | - | GENCODE basic, APPRIS P1, |
| CSNK2A1-244 | ENST00000646814.1 | 2812 | 391aa | Protein coding | CCDS13003 | P68400-1 | - | GENCODE basic, APPRIS P1, |
| CSNK2A1-242 | ENST00000646477.1 | 2624 | 255aa | Protein coding | CCDS13004 | P68400-2 | - | GENCODE basic, |
| CSNK2A1-223 | ENST00000644003.1 | 2319 | 255aa | Protein coding | CCDS13004 | P68400-2 | - | GENCODE basic, |
| CSNK2A1-235 | ENST00000645623.1 | 2192 | 391aa | Protein coding | CCDS13003 | P68400-1 | - | GENCODE basic, APPRIS P1, |
| CSNK2A1-248 | ENST00000647348.1 | 1781 | 391aa | Protein coding | CCDS13003 | P68400-1 | - | GENCODE basic, APPRIS P1, |
| CSNK2A1-203 | ENST00000400217.7 | 1485 | 391aa | Protein coding | CCDS13003 | P68400-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| CSNK2A1-233 | ENST00000645260.1 | 3568 | 354aa | Protein coding | - | A0A2R8YDP2 | - | GENCODE basic, |
| CSNK2A1-227 | ENST00000644710.1 | 2758 | 374aa | Protein coding | - | A0A2R8YFU2 | - | GENCODE basic, |
| CSNK2A1-231 | ENST00000645234.1 | 2586 | 376aa | Protein coding | - | A0A2R8YD58 | - | GENCODE basic, |
| CSNK2A1-213 | ENST00000642689.1 | 2545 | 374aa | Protein coding | - | A0A2R8YFU2 | - | GENCODE basic, |
| CSNK2A1-228 | ENST00000644885.1 | 2479 | 363aa | Protein coding | - | A0A2R8YF43 | - | GENCODE basic, |
| CSNK2A1-246 | ENST00000647026.1 | 1806 | 354aa | Protein coding | - | A0A2R8Y5A0 | - | GENCODE basic, |
| CSNK2A1-204 | ENST00000400227.8 | 1490 | 385aa | Protein coding | - | E7EU96 | - | TSL:1, GENCODE basic, |
| CSNK2A1-211 | ENST00000642160.1 | 1419 | 86aa | Protein coding | - | A0A2R8Y797 | - | GENCODE basic, |
| CSNK2A1-214 | ENST00000643600.1 | 1156 | 256aa | Protein coding | - | A0A2R8Y4H0 | - | GENCODE basic, |
| CSNK2A1-245 | ENST00000646908.1 | 1144 | 74aa | Protein coding | - | A0A2R8Y3W6 | - | CDS 3' incomplete, |
| CSNK2A1-210 | ENST00000619801.5 | 909 | 118aa | Protein coding | - | A0A087WY74 | - | CDS 5' incomplete, TSL:3, |
| CSNK2A1-218 | ENST00000643680.1 | 849 | 207aa | Protein coding | - | A0A2R8Y4D6 | - | CDS 3' incomplete, |
| CSNK2A1-208 | ENST00000609525.2 | 846 | 144aa | Protein coding | - | V9GYA2 | - | CDS 3' incomplete, TSL:5, |
| CSNK2A1-230 | ENST00000645187.1 | 821 | 152aa | Protein coding | - | A0A2R8Y7T1 | - | GENCODE basic, |
| CSNK2A1-205 | ENST00000460062.7 | 651 | 42aa | Protein coding | - | V9GYW6 | - | CDS 3' incomplete, TSL:4, |
| CSNK2A1-206 | ENST00000608066.6 | 616 | 92aa | Protein coding | - | V9GY80 | - | CDS 3' incomplete, TSL:4, |
| CSNK2A1-225 | ENST00000644177.1 | 600 | 131aa | Protein coding | - | A0A2R8YDY7 | - | CDS 3' incomplete, |
| CSNK2A1-220 | ENST00000643910.1 | 546 | 33aa | Protein coding | - | A0A2R8YCC9 | - | CDS 3' incomplete, |
| CSNK2A1-229 | ENST00000645091.1 | 526 | 133aa | Protein coding | - | A0A2R8YEW1 | - | CDS 3' incomplete, |
| CSNK2A1-238 | ENST00000645840.1 | 4295 | 88aa | Nonsense mediated decay | - | A0A2R8YEL7 | - | - |
| CSNK2A1-239 | ENST00000645910.1 | 4290 | 117aa | Nonsense mediated decay | - | A0A2R8YCK2 | - | - |
| CSNK2A1-232 | ENST00000645249.1 | 2860 | 94aa | Nonsense mediated decay | - | A0A2R8YF47 | - | - |
| CSNK2A1-221 | ENST00000643968.1 | 2346 | 117aa | Nonsense mediated decay | - | A0A2R8YCK2 | - | - |
| CSNK2A1-224 | ENST00000644170.1 | 5121 | No protein | Retained intron | - | - | - | - |
| CSNK2A1-219 | ENST00000643700.1 | 4667 | No protein | Retained intron | - | - | - | - |
| CSNK2A1-247 | ENST00000647155.1 | 3622 | No protein | Retained intron | - | - | - | - |
| CSNK2A1-222 | ENST00000643980.1 | 3253 | No protein | Retained intron | - | - | - | - |
| CSNK2A1-237 | ENST00000645768.1 | 2899 | No protein | Retained intron | - | - | - | - |
| CSNK2A1-226 | ENST00000644448.1 | 2895 | No protein | Retained intron | - | - | - | - |
| CSNK2A1-207 | ENST00000608490.2 | 2807 | No protein | Retained intron | - | - | - | TSL:2, |
| CSNK2A1-236 | ENST00000645641.1 | 2681 | No protein | Retained intron | - | - | - | - |
| CSNK2A1-216 | ENST00000643641.1 | 2451 | No protein | Retained intron | - | - | - | - |
| CSNK2A1-212 | ENST00000642673.1 | 2151 | No protein | Retained intron | - | - | - | - |
| CSNK2A1-234 | ENST00000645334.1 | 2123 | No protein | Retained intron | - | - | - | - |
| CSNK2A1-215 | ENST00000643602.1 | 2023 | No protein | Retained intron | - | - | - | - |
| CSNK2A1-209 | ENST00000609606.6 | 2018 | No protein | Retained intron | - | - | - | TSL:2, |
| CSNK2A1-241 | ENST00000646443.1 | 1598 | No protein | Retained intron | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CSNK2A1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CSNK2A1 had been KO in hct116 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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