CRISPR-U™ Gene Knockout Cell Line Strategy

CSNK1D Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CSNK1D Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CSNK1D
Gene id 1453
Organism Homo sapiens
Official full symbol casein kinase 1 delta
Gene type protein-coding
Also known as ASPS, CKI-delta, CKId, CKIdelta, FASPS2, HCKID
Summary This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 17
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CSNK1D-202 ENST00000314028.10 3712 415aa Protein coding CCDS11805 P48730-1 - TSL:1, GENCODE basic, APPRIS ALT1,
CSNK1D-203 ENST00000392334.6 2047 409aa Protein coding CCDS11806 P48730-2 - TSL:1, GENCODE basic, APPRIS P4,
CSNK1D-204 ENST00000398519.9 1580 427aa Protein coding CCDS86654 H7BYT1 - TSL:5, GENCODE basic,
CSNK1D-213 ENST00000580446.1 612 187aa Protein coding - J3QQI9 - CDS 3' incomplete, TSL:3,
CSNK1D-201 ENST00000269361.11 1088 120aa Nonsense mediated decay - H0Y2N6 - CDS 5' incomplete, TSL:5,
CSNK1D-215 ENST00000580784.5 1081 20aa Nonsense mediated decay - J3QQU8 - CDS 5' incomplete, TSL:1,
CSNK1D-205 ENST00000403276.7 1040 126aa Nonsense mediated decay - B4DLF1 - TSL:2,
CSNK1D-219 ENST00000581660.5 611 43aa Nonsense mediated decay - J3QS70 - TSL:5,
CSNK1D-226 ENST00000585026.1 563 56aa Nonsense mediated decay - J3KRS6 - TSL:5,
CSNK1D-224 ENST00000584672.1 401 64aa Nonsense mediated decay - J3KRM8 - CDS 5' incomplete, TSL:3,
CSNK1D-209 ENST00000578904.1 1269 No protein Processed transcript - - - TSL:3,
CSNK1D-221 ENST00000582844.5 957 No protein Processed transcript - - - TSL:5,
CSNK1D-206 ENST00000577578.5 928 No protein Processed transcript - - - TSL:2,
CSNK1D-223 ENST00000584472.5 586 No protein Processed transcript - - - TSL:4,
CSNK1D-207 ENST00000578194.5 567 No protein Processed transcript - - - TSL:4,
CSNK1D-214 ENST00000580565.1 566 No protein Processed transcript - - - TSL:4,
CSNK1D-218 ENST00000581655.1 549 No protein Processed transcript - - - TSL:4,
CSNK1D-225 ENST00000584913.1 295 No protein Processed transcript - - - TSL:2,
CSNK1D-217 ENST00000581241.5 6094 No protein Retained intron - - - TSL:2,
CSNK1D-222 ENST00000584377.5 2891 No protein Retained intron - - - TSL:2,
CSNK1D-208 ENST00000578501.5 1124 No protein Retained intron - - - TSL:2,
CSNK1D-211 ENST00000579316.5 692 No protein Retained intron - - - TSL:2,
CSNK1D-220 ENST00000581737.1 631 No protein Retained intron - - - TSL:2,
CSNK1D-216 ENST00000581108.5 607 No protein Retained intron - - - TSL:2,
CSNK1D-210 ENST00000579308.1 598 No protein Retained intron - - - TSL:2,
CSNK1D-212 ENST00000580061.5 578 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CSNK1D
This KO Strategy loading
Red Cotton™ Notes Gene CSNK1D had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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