CRISPR-U™ Gene Knockout Cell Line
Strategy
CSNK1D Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CSNK1D Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CSNK1D |
| Gene id | 1453 |
| Organism | Homo sapiens |
| Official full symbol | casein kinase 1 delta |
| Gene type | protein-coding |
| Also known as | ASPS, CKI-delta, CKId, CKIdelta, FASPS2, HCKID |
| Summary | This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CSNK1D-202 | ENST00000314028.10 | 3712 | 415aa | Protein coding | CCDS11805 | P48730-1 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| CSNK1D-203 | ENST00000392334.6 | 2047 | 409aa | Protein coding | CCDS11806 | P48730-2 | - | TSL:1, GENCODE basic, APPRIS P4, |
| CSNK1D-204 | ENST00000398519.9 | 1580 | 427aa | Protein coding | CCDS86654 | H7BYT1 | - | TSL:5, GENCODE basic, |
| CSNK1D-213 | ENST00000580446.1 | 612 | 187aa | Protein coding | - | J3QQI9 | - | CDS 3' incomplete, TSL:3, |
| CSNK1D-201 | ENST00000269361.11 | 1088 | 120aa | Nonsense mediated decay | - | H0Y2N6 | - | CDS 5' incomplete, TSL:5, |
| CSNK1D-215 | ENST00000580784.5 | 1081 | 20aa | Nonsense mediated decay | - | J3QQU8 | - | CDS 5' incomplete, TSL:1, |
| CSNK1D-205 | ENST00000403276.7 | 1040 | 126aa | Nonsense mediated decay | - | B4DLF1 | - | TSL:2, |
| CSNK1D-219 | ENST00000581660.5 | 611 | 43aa | Nonsense mediated decay | - | J3QS70 | - | TSL:5, |
| CSNK1D-226 | ENST00000585026.1 | 563 | 56aa | Nonsense mediated decay | - | J3KRS6 | - | TSL:5, |
| CSNK1D-224 | ENST00000584672.1 | 401 | 64aa | Nonsense mediated decay | - | J3KRM8 | - | CDS 5' incomplete, TSL:3, |
| CSNK1D-209 | ENST00000578904.1 | 1269 | No protein | Processed transcript | - | - | - | TSL:3, |
| CSNK1D-221 | ENST00000582844.5 | 957 | No protein | Processed transcript | - | - | - | TSL:5, |
| CSNK1D-206 | ENST00000577578.5 | 928 | No protein | Processed transcript | - | - | - | TSL:2, |
| CSNK1D-223 | ENST00000584472.5 | 586 | No protein | Processed transcript | - | - | - | TSL:4, |
| CSNK1D-207 | ENST00000578194.5 | 567 | No protein | Processed transcript | - | - | - | TSL:4, |
| CSNK1D-214 | ENST00000580565.1 | 566 | No protein | Processed transcript | - | - | - | TSL:4, |
| CSNK1D-218 | ENST00000581655.1 | 549 | No protein | Processed transcript | - | - | - | TSL:4, |
| CSNK1D-225 | ENST00000584913.1 | 295 | No protein | Processed transcript | - | - | - | TSL:2, |
| CSNK1D-217 | ENST00000581241.5 | 6094 | No protein | Retained intron | - | - | - | TSL:2, |
| CSNK1D-222 | ENST00000584377.5 | 2891 | No protein | Retained intron | - | - | - | TSL:2, |
| CSNK1D-208 | ENST00000578501.5 | 1124 | No protein | Retained intron | - | - | - | TSL:2, |
| CSNK1D-211 | ENST00000579316.5 | 692 | No protein | Retained intron | - | - | - | TSL:2, |
| CSNK1D-220 | ENST00000581737.1 | 631 | No protein | Retained intron | - | - | - | TSL:2, |
| CSNK1D-216 | ENST00000581108.5 | 607 | No protein | Retained intron | - | - | - | TSL:2, |
| CSNK1D-210 | ENST00000579308.1 | 598 | No protein | Retained intron | - | - | - | TSL:2, |
| CSNK1D-212 | ENST00000580061.5 | 578 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CSNK1D |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CSNK1D had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
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$599
Single gRNA lentivirus
Work flow
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