CRISPR-U™ Gene Knockout Cell Line Strategy

CSF1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CSF1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CSF1
Gene id 1435
Organism Homo sapiens
Official full symbol colony stimulating factor 1
Gene type protein-coding
Also known as CSF-1, MCSF
Summary The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants.
Genomic regions Chromosome 1
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CSF1-201 ENST00000329608.11 3994 554aa Protein coding CCDS816 P09603-1 NM_000757.6 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CSF1-204 ENST00000369802.7 2484 554aa Protein coding CCDS816 P09603-1 - TSL:1, GENCODE basic, APPRIS P4,
CSF1-203 ENST00000369801.1 1364 438aa Protein coding CCDS817 P09603-2 - TSL:1, GENCODE basic, APPRIS ALT2,
CSF1-205 ENST00000420111.6 1083 256aa Protein coding CCDS30797 P09603-3 - TSL:5, GENCODE basic, APPRIS ALT2,
CSF1-206 ENST00000488198.5 1009 285aa Protein coding - E9PJA2 - CDS 3' incomplete, TSL:3,
CSF1-202 ENST00000357302.8 597 119aa Protein coding - H7BY18 - CDS 3' incomplete, TSL:3,
CSF1-209 ENST00000527192.5 577 150aa Protein coding - E9PQ08 - CDS 3' incomplete, TSL:4,
CSF1-207 ENST00000525659.5 551 113aa Protein coding - E9PKP4 - CDS 3' incomplete, TSL:4,
CSF1-208 ENST00000526001.1 573 No protein Processed transcript - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CSF1
This KO Strategy loading
Red Cotton™ Notes Gene CSF1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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