CRISPR-U™ Gene Knockout Cell Line
Strategy
CSF1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CSF1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CSF1 |
| Gene id | 1435 |
| Organism | Homo sapiens |
| Official full symbol | colony stimulating factor 1 |
| Gene type | protein-coding |
| Also known as | CSF-1, MCSF |
| Summary | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CSF1-201 | ENST00000329608.11 | 3994 | 554aa | Protein coding | CCDS816 | P09603-1 | NM_000757.6 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| CSF1-204 | ENST00000369802.7 | 2484 | 554aa | Protein coding | CCDS816 | P09603-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| CSF1-203 | ENST00000369801.1 | 1364 | 438aa | Protein coding | CCDS817 | P09603-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| CSF1-205 | ENST00000420111.6 | 1083 | 256aa | Protein coding | CCDS30797 | P09603-3 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| CSF1-206 | ENST00000488198.5 | 1009 | 285aa | Protein coding | - | E9PJA2 | - | CDS 3' incomplete, TSL:3, |
| CSF1-202 | ENST00000357302.8 | 597 | 119aa | Protein coding | - | H7BY18 | - | CDS 3' incomplete, TSL:3, |
| CSF1-209 | ENST00000527192.5 | 577 | 150aa | Protein coding | - | E9PQ08 | - | CDS 3' incomplete, TSL:4, |
| CSF1-207 | ENST00000525659.5 | 551 | 113aa | Protein coding | - | E9PKP4 | - | CDS 3' incomplete, TSL:4, |
| CSF1-208 | ENST00000526001.1 | 573 | No protein | Processed transcript | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CSF1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CSF1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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