CRISPR-U™ Gene Knockout Cell Line Strategy

PARP1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PARP1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PARP1
Gene id 142
Organism Homo sapiens
Official full symbol poly(ADP-ribose) polymerase 1
Gene type protein-coding
Also known as ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1
Summary This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PARP1-203 ENST00000366794.10 3978 1014aa Protein coding CCDS1554 A0A024R3T8 P09874 NM_001618.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PARP1-216 ENST00000677203.1 3850 971aa Protein coding - - - GENCODE basic,
PARP1-224 ENST00000678560.1 4255 100aa Nonsense mediated decay - - - -
PARP1-214 ENST00000677091.1 3768 369aa Nonsense mediated decay - - - -
PARP1-221 ENST00000678144.1 3719 308aa Nonsense mediated decay - - - -
PARP1-222 ENST00000678226.1 3274 No protein Processed transcript - - - -
PARP1-215 ENST00000677189.1 2348 No protein Processed transcript - - - -
PARP1-220 ENST00000677985.1 2285 No protein Processed transcript - - - -
PARP1-210 ENST00000676481.1 1371 No protein Processed transcript - - - -
PARP1-202 ENST00000366792.3 553 No protein Processed transcript - - - TSL:3,
PARP1-206 ENST00000469663.1 542 No protein Processed transcript - - - TSL:3,
PARP1-212 ENST00000676685.1 6210 No protein Retained intron - - - -
PARP1-217 ENST00000677374.1 5697 No protein Retained intron - - - -
PARP1-209 ENST00000498787.2 5629 No protein Retained intron - - - TSL:2,
PARP1-225 ENST00000678781.1 5430 No protein Retained intron - - - -
PARP1-226 ENST00000679276.1 4918 No protein Retained intron - - - -
PARP1-213 ENST00000676709.1 4764 No protein Retained intron - - - -
PARP1-219 ENST00000677884.1 4620 No protein Retained intron - - - -
PARP1-218 ENST00000677815.1 3770 No protein Retained intron - - - -
PARP1-211 ENST00000676565.1 3577 No protein Retained intron - - - -
PARP1-201 ENST00000366790.4 3358 No protein Retained intron - - - TSL:2,
PARP1-207 ENST00000490921.5 3165 No protein Retained intron - - - TSL:2,
PARP1-223 ENST00000678288.1 2420 No protein Retained intron - - - -
PARP1-204 ENST00000463968.5 830 No protein Retained intron - - - TSL:2,
PARP1-205 ENST00000468608.1 438 No protein Retained intron - - - TSL:3,
PARP1-208 ENST00000491816.1 416 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PARP1
This KO Strategy loading
Red Cotton™ Notes Gene PARP1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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