CRISPR-U™ Gene Knockout Cell Line Strategy

ISM1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ISM1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ISM1
Gene id 140862
Organism Homo sapiens
Official full symbol isthmin 1
Gene type protein-coding
Also known as C20orf82, ISM, Isthmin, bA149I18.1, dJ1077I2.1
Genomic regions Chromosome 20
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ISM1-201 ENST00000262487.5 3090 464aa Protein coding CCDS46579 B1AKI9 NM_080826.2 TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ISM1
This KO Strategy loading
Red Cotton™ Notes Gene ISM1 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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