CRISPR-U™ Gene Knockout Cell Line
Strategy
ISM1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ISM1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ISM1 |
| Gene id | 140862 |
| Organism | Homo sapiens |
| Official full symbol | isthmin 1 |
| Gene type | protein-coding |
| Also known as | C20orf82, ISM, Isthmin, bA149I18.1, dJ1077I2.1 |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ISM1-201 | ENST00000262487.5 | 3090 | 464aa | Protein coding | CCDS46579 | B1AKI9 | NM_080826.2 | TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ISM1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ISM1 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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