CRISPR-U™ Gene Knockout Cell Line
Strategy
CRAT Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CRAT Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CRAT |
| Gene id | 1384 |
| Organism | Homo sapiens |
| Official full symbol | carnitine O-acetyltransferase |
| Gene type | protein-coding |
| Also known as | CAT, CAT1, NBIA8 |
| Summary | This gene encodes carnitine O-acetyltransferase, a member of the carnitine acyltransferase family and a key metabolic pathway enzyme which plays an important role in energy homeostasis and fat metabolism. This enzyme catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acyl-CoA/CoA. It is found in both the mitochondria and the peroxisome. Alternative splicing results in transcript variants encoding different isoforms that may localize to different subcellular compartments. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CRAT-201 | ENST00000318080.7 | 2768 | 626aa | Protein coding | CCDS6919 | P43155-1 | NM_000755.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CRAT-219 | ENST00000681627.1 | 2623 | 602aa | Protein coding | - | - | - | GENCODE basic, |
| CRAT-213 | ENST00000680117.1 | 2618 | 590aa | Protein coding | - | - | - | GENCODE basic, |
| CRAT-217 | ENST00000681325.1 | 2529 | 551aa | Protein coding | - | - | - | GENCODE basic, |
| CRAT-205 | ENST00000455396.2 | 2524 | 586aa | Protein coding | - | H0Y4Z7 | - | TSL:3, GENCODE basic, |
| CRAT-221 | ENST00000681725.1 | 2107 | 572aa | Protein coding | - | - | - | GENCODE basic, |
| CRAT-202 | ENST00000393384.3 | 973 | 161aa | Protein coding | - | A6PVN3 | - | TSL:2, GENCODE basic, |
| CRAT-206 | ENST00000455830.2 | 595 | 98aa | Protein coding | - | B7ZBP5 | - | CDS 3' incomplete, TSL:5, |
| CRAT-218 | ENST00000681622.1 | 3089 | 40aa | Nonsense mediated decay | - | - | - | - |
| CRAT-210 | ENST00000679520.1 | 2848 | 40aa | Nonsense mediated decay | - | - | - | - |
| CRAT-222 | ENST00000681911.1 | 2831 | 40aa | Nonsense mediated decay | - | - | - | - |
| CRAT-215 | ENST00000681040.1 | 2767 | 40aa | Nonsense mediated decay | - | - | - | - |
| CRAT-220 | ENST00000681689.1 | 2562 | 40aa | Nonsense mediated decay | - | - | - | - |
| CRAT-212 | ENST00000680093.1 | 2539 | 212aa | Nonsense mediated decay | - | - | - | - |
| CRAT-207 | ENST00000458362.5 | 2253 | 40aa | Nonsense mediated decay | - | F2Z2C5 | - | TSL:1, |
| CRAT-203 | ENST00000415948.6 | 1029 | 40aa | Nonsense mediated decay | - | H7C0E1 | - | TSL:5, |
| CRAT-204 | ENST00000441796.1 | 831 | 40aa | Nonsense mediated decay | - | F2Z2C5 | - | TSL:5, |
| CRAT-208 | ENST00000464290.5 | 867 | No protein | Processed transcript | - | - | - | TSL:3, |
| CRAT-209 | ENST00000467343.1 | 399 | No protein | Processed transcript | - | - | - | TSL:5, |
| CRAT-211 | ENST00000679716.1 | 4466 | No protein | Retained intron | - | - | - | - |
| CRAT-214 | ENST00000680523.1 | 4118 | No protein | Retained intron | - | - | - | - |
| CRAT-216 | ENST00000681118.1 | 3938 | No protein | Retained intron | - | - | - | - |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CRAT |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CRAT had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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