CRISPR-U™ Gene Knockout Cell Line
Strategy
MPLKIP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MPLKIP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MPLKIP |
| Gene id | 136647 |
| Organism | Homo sapiens |
| Official full symbol | M-phase specific PLK1 interacting protein |
| Gene type | protein-coding |
| Also known as | ABHS, C7orf11, ORF20, TTD4 |
| Summary | The protein encoded by this gene localizes to the centrosome during mitosis and to the midbody during cytokinesis. The protein is phosphorylated by cyclin-dependent kinase 1 during mitosis and subsequently interacts with polo-like kinase 1. The protein is thought to function in regulating mitosis and cytokinesis. Mutations in this gene result in nonphotosensitive trichothiodystrophy. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MPLKIP-201 | ENST00000306984.8 | 7627 | 179aa | Protein coding | CCDS5463 | A4D1W6 Q8TAP9 | NM_138701.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MPLKIP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MPLKIP had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: