CRISPR-U™ Gene Knockout Cell Line
Strategy
ADORA2B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ADORA2B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ADORA2B |
| Gene id | 136 |
| Organism | Homo sapiens |
| Official full symbol | adenosine A2b receptor |
| Gene type | protein-coding |
| Also known as | ADORA2 |
| Summary | This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ADORA2B-201 | ENST00000304222.2 | 1735 | 332aa | Protein coding | CCDS11173 | P29275 | - | TSL:1, GENCODE basic, APPRIS P1, |
| ADORA2B-202 | ENST00000582124.1 | 1875 | No protein | Retained intron | - | - | - | TSL:NA, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ADORA2B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ADORA2B had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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