CRISPR-U™ Gene Knockout Cell Line
Strategy
NCOA7 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NCOA7 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NCOA7 |
| Gene id | 135112 |
| Organism | Homo sapiens |
| Official full symbol | nuclear receptor coactivator 7 |
| Gene type | protein-coding |
| Also known as | ERAP140, ESNA1, NCOA7-AS, Nbla00052, Nbla10993, TLDC4, dJ187J11.3 |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NCOA7-203 | ENST00000392477.7 | 6264 | 942aa | Protein coding | CCDS5132 | Q8NI08-1 | NM_181782.5 | TSL:1, GENCODE basic, MANE Select v0.92, |
| NCOA7-202 | ENST00000368357.7 | 5521 | 942aa | Protein coding | CCDS5132 | Q8NI08-1 | - | TSL:1, GENCODE basic, |
| NCOA7-208 | ENST00000438495.6 | 3133 | 219aa | Protein coding | CCDS83125 | Q8NI08-5 | - | TSL:1, GENCODE basic, APPRIS P1, |
| NCOA7-201 | ENST00000229634.13 | 2998 | 827aa | Protein coding | CCDS56448 | Q8NI08-7 | - | TSL:2, GENCODE basic, |
| NCOA7-209 | ENST00000444128.2 | 2978 | 159aa | Protein coding | - | H0Y6T0 | - | TSL:5, GENCODE basic, |
| NCOA7-204 | ENST00000417494.5 | 765 | 191aa | Protein coding | - | Q5TF96 | - | CDS 3' incomplete, TSL:2, |
| NCOA7-210 | ENST00000453302.5 | 581 | 90aa | Protein coding | - | Q5TF98 | - | CDS 3' incomplete, TSL:3, |
| NCOA7-206 | ENST00000428318.1 | 548 | 90aa | Protein coding | - | Q5TF98 | - | CDS 3' incomplete, TSL:3, |
| NCOA7-205 | ENST00000419660.1 | 488 | 117aa | Protein coding | - | Q5TF97 | - | CDS 3' incomplete, TSL:5, |
| NCOA7-211 | ENST00000487635.1 | 758 | No protein | Processed transcript | - | - | - | TSL:1, |
| NCOA7-207 | ENST00000433571.2 | 636 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NCOA7 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NCOA7 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: