CRISPR-U™ Gene Knockout Cell Line Strategy

NCOA7 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NCOA7 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NCOA7
Gene id 135112
Organism Homo sapiens
Official full symbol nuclear receptor coactivator 7
Gene type protein-coding
Also known as ERAP140, ESNA1, NCOA7-AS, Nbla00052, Nbla10993, TLDC4, dJ187J11.3
Genomic regions Chromosome 6
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NCOA7-203 ENST00000392477.7 6264 942aa Protein coding CCDS5132 Q8NI08-1 NM_181782.5 TSL:1, GENCODE basic, MANE Select v0.92,
NCOA7-202 ENST00000368357.7 5521 942aa Protein coding CCDS5132 Q8NI08-1 - TSL:1, GENCODE basic,
NCOA7-208 ENST00000438495.6 3133 219aa Protein coding CCDS83125 Q8NI08-5 - TSL:1, GENCODE basic, APPRIS P1,
NCOA7-201 ENST00000229634.13 2998 827aa Protein coding CCDS56448 Q8NI08-7 - TSL:2, GENCODE basic,
NCOA7-209 ENST00000444128.2 2978 159aa Protein coding - H0Y6T0 - TSL:5, GENCODE basic,
NCOA7-204 ENST00000417494.5 765 191aa Protein coding - Q5TF96 - CDS 3' incomplete, TSL:2,
NCOA7-210 ENST00000453302.5 581 90aa Protein coding - Q5TF98 - CDS 3' incomplete, TSL:3,
NCOA7-206 ENST00000428318.1 548 90aa Protein coding - Q5TF98 - CDS 3' incomplete, TSL:3,
NCOA7-205 ENST00000419660.1 488 117aa Protein coding - Q5TF97 - CDS 3' incomplete, TSL:5,
NCOA7-211 ENST00000487635.1 758 No protein Processed transcript - - - TSL:1,
NCOA7-207 ENST00000433571.2 636 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NCOA7
This KO Strategy loading
Red Cotton™ Notes Gene NCOA7 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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