CRISPR-U™ Gene Knockout Cell Line Strategy

COX7B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human COX7B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol COX7B
Gene id 1349
Organism Homo sapiens
Official full symbol cytochrome c oxidase subunit 7B
Gene type protein-coding
Also known as APLCC, LSDMCA2
Summary Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes subunit VIIb, which is highly similar to bovine COX VIIb protein and is found in all tissues. This gene may have several pseudogenes on chromosomes 1, 2, 20 and 22.
Genomic regions Chromosome X
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
COX7B-204 ENST00000650309.2 2444 80aa Protein coding CCDS14437 P24311 NM_001866.3 GENCODE basic, APPRIS P1, MANE Select v0.92,
COX7B-202 ENST00000475465.1 685 60aa Protein coding - A0A3B3ITX0 - TSL:2, GENCODE basic,
COX7B-201 ENST00000373335.4 605 60aa Protein coding - A0A3B3IRN8 - TSL:2, GENCODE basic,
COX7B-203 ENST00000647835.1 505 39aa Protein coding - A0A3B3ISY5 - GENCODE basic,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene COX7B
This KO Strategy loading
Red Cotton™ Notes Gene COX7B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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