CRISPR-U™ Gene Knockout Cell Line
Strategy
COX7B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human COX7B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | COX7B |
| Gene id | 1349 |
| Organism | Homo sapiens |
| Official full symbol | cytochrome c oxidase subunit 7B |
| Gene type | protein-coding |
| Also known as | APLCC, LSDMCA2 |
| Summary | Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes subunit VIIb, which is highly similar to bovine COX VIIb protein and is found in all tissues. This gene may have several pseudogenes on chromosomes 1, 2, 20 and 22. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| COX7B-204 | ENST00000650309.2 | 2444 | 80aa | Protein coding | CCDS14437 | P24311 | NM_001866.3 | GENCODE basic, APPRIS P1, MANE Select v0.92, |
| COX7B-202 | ENST00000475465.1 | 685 | 60aa | Protein coding | - | A0A3B3ITX0 | - | TSL:2, GENCODE basic, |
| COX7B-201 | ENST00000373335.4 | 605 | 60aa | Protein coding | - | A0A3B3IRN8 | - | TSL:2, GENCODE basic, |
| COX7B-203 | ENST00000647835.1 | 505 | 39aa | Protein coding | - | A0A3B3ISY5 | - | GENCODE basic, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | COX7B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene COX7B had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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