CRISPR-U™ Gene Knockout Cell Line
Strategy
COX7A2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human COX7A2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | COX7A2 |
| Gene id | 1347 |
| Organism | Homo sapiens |
| Official full symbol | cytochrome c oxidase subunit 7A2 |
| Gene type | protein-coding |
| Also known as | COX7AL, COX7AL1, COXVIIAL, COXVIIa-L, VIIAL |
| Summary | Cytochrome c oxidase, the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of three catalytic subunits encoded by mitochondrial genes, and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, while the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (liver isoform) of subunit VIIa, with this polypeptide being present in both muscle and non-muscle tissues. In addition to polypeptide 2, subunit VIIa includes polypeptide 1 (muscle isoform), which is present only in muscle tissues, and a related protein, which is present in all tissues. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4 and 14. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| COX7A2-201 | ENST00000370081.6 | 914 | 115aa | Protein coding | CCDS34486 | H0UI06 | - | TSL:3, GENCODE basic, APPRIS P1, |
| COX7A2-202 | ENST00000370089.6 | 773 | 115aa | Protein coding | CCDS34486 | H0UI06 | - | TSL:1, GENCODE basic, APPRIS P1, |
| COX7A2-208 | ENST00000509698.5 | 480 | 91aa | Protein coding | - | D6RIE3 | - | TSL:3, GENCODE basic, |
| COX7A2-203 | ENST00000377978.3 | 366 | 103aa | Protein coding | - | D6RGV5 | - | CDS 3' incomplete, TSL:5, |
| COX7A2-205 | ENST00000460985.1 | 357 | 53aa | Protein coding | - | D6R9C3 | - | TSL:2, GENCODE basic, |
| COX7A2-206 | ENST00000472311.6 | 325 | 76aa | Protein coding | - | D6RA35 | - | TSL:2, GENCODE basic, |
| COX7A2-204 | ENST00000459637.2 | 501 | 67aa | Nonsense mediated decay | - | H0Y8S6 | - | CDS 5' incomplete, TSL:2, |
| COX7A2-207 | ENST00000481061.2 | 914 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | COX7A2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene COX7A2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
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$599
Single gRNA lentivirus
Work flow
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