CRISPR-U™ Gene Knockout Cell Line Strategy

COX7A2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human COX7A2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol COX7A2
Gene id 1347
Organism Homo sapiens
Official full symbol cytochrome c oxidase subunit 7A2
Gene type protein-coding
Summary Cytochrome c oxidase, the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of three catalytic subunits encoded by mitochondrial genes, and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, while the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (liver isoform) of subunit VIIa, with this polypeptide being present in both muscle and non-muscle tissues. In addition to polypeptide 2, subunit VIIa includes polypeptide 1 (muscle isoform), which is present only in muscle tissues, and a related protein, which is present in all tissues. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4 and 14.
Genomic regions Chromosome 6
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
COX7A2-201 ENST00000370081.6 914 115aa Protein coding CCDS34486 H0UI06 - TSL:3, GENCODE basic, APPRIS P1,
COX7A2-202 ENST00000370089.6 773 115aa Protein coding CCDS34486 H0UI06 - TSL:1, GENCODE basic, APPRIS P1,
COX7A2-208 ENST00000509698.5 480 91aa Protein coding - D6RIE3 - TSL:3, GENCODE basic,
COX7A2-203 ENST00000377978.3 366 103aa Protein coding - D6RGV5 - CDS 3' incomplete, TSL:5,
COX7A2-205 ENST00000460985.1 357 53aa Protein coding - D6R9C3 - TSL:2, GENCODE basic,
COX7A2-206 ENST00000472311.6 325 76aa Protein coding - D6RA35 - TSL:2, GENCODE basic,
COX7A2-204 ENST00000459637.2 501 67aa Nonsense mediated decay - H0Y8S6 - CDS 5' incomplete, TSL:2,
COX7A2-207 ENST00000481061.2 914 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene COX7A2
This KO Strategy loading
Red Cotton™ Notes Gene COX7A2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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