CRISPR-U™ Gene Knockout Cell Line
Strategy
COL9A3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human COL9A3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | COL9A3 |
| Gene id | 1299 |
| Organism | Homo sapiens |
| Official full symbol | collagen type IX alpha 3 chain |
| Gene type | protein-coding |
| Also known as | DJ885L7.4.1, EDM3, IDD, MED |
| Summary | This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. Mutations in this gene are associated with multiple epiphyseal dysplasia type 3. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| COL9A3-214 | ENST00000649368.1 | 2497 | 684aa | Protein coding | CCDS13505 | Q14050 | NM_001853.4 | GENCODE basic, APPRIS P1, MANE Select v0.92, |
| COL9A3-201 | ENST00000452372.2 | 617 | 191aa | Protein coding | - | Q4VXW1 | - | CDS 3' incomplete, TSL:5, |
| COL9A3-202 | ENST00000462700.5 | 1237 | No protein | Processed transcript | - | - | - | TSL:3, |
| COL9A3-206 | ENST00000467819.5 | 993 | No protein | Processed transcript | - | - | - | TSL:1, |
| COL9A3-212 | ENST00000489045.5 | 838 | No protein | Processed transcript | - | - | - | TSL:5, |
| COL9A3-203 | ENST00000463487.2 | 716 | No protein | Processed transcript | - | - | - | TSL:5, |
| COL9A3-210 | ENST00000477612.5 | 635 | No protein | Processed transcript | - | - | - | TSL:3, |
| COL9A3-205 | ENST00000466532.1 | 539 | No protein | Processed transcript | - | - | - | TSL:2, |
| COL9A3-204 | ENST00000466192.5 | 2194 | No protein | Retained intron | - | - | - | TSL:2, |
| COL9A3-209 | ENST00000472880.1 | 959 | No protein | Retained intron | - | - | - | TSL:3, |
| COL9A3-208 | ENST00000469852.5 | 901 | No protein | Retained intron | - | - | - | TSL:3, |
| COL9A3-207 | ENST00000469802.5 | 680 | No protein | Retained intron | - | - | - | TSL:2, |
| COL9A3-211 | ENST00000481800.1 | 548 | No protein | Retained intron | - | - | - | TSL:4, |
| COL9A3-213 | ENST00000490398.5 | 496 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | COL9A3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene COL9A3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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