CRISPR-U™ Gene Knockout Cell Line Strategy

COL9A3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human COL9A3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol COL9A3
Gene id 1299
Organism Homo sapiens
Official full symbol collagen type IX alpha 3 chain
Gene type protein-coding
Also known as DJ885L7.4.1, EDM3, IDD, MED
Summary This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. Mutations in this gene are associated with multiple epiphyseal dysplasia type 3.
Genomic regions Chromosome 20
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
COL9A3-214 ENST00000649368.1 2497 684aa Protein coding CCDS13505 Q14050 NM_001853.4 GENCODE basic, APPRIS P1, MANE Select v0.92,
COL9A3-201 ENST00000452372.2 617 191aa Protein coding - Q4VXW1 - CDS 3' incomplete, TSL:5,
COL9A3-202 ENST00000462700.5 1237 No protein Processed transcript - - - TSL:3,
COL9A3-206 ENST00000467819.5 993 No protein Processed transcript - - - TSL:1,
COL9A3-212 ENST00000489045.5 838 No protein Processed transcript - - - TSL:5,
COL9A3-203 ENST00000463487.2 716 No protein Processed transcript - - - TSL:5,
COL9A3-210 ENST00000477612.5 635 No protein Processed transcript - - - TSL:3,
COL9A3-205 ENST00000466532.1 539 No protein Processed transcript - - - TSL:2,
COL9A3-204 ENST00000466192.5 2194 No protein Retained intron - - - TSL:2,
COL9A3-209 ENST00000472880.1 959 No protein Retained intron - - - TSL:3,
COL9A3-208 ENST00000469852.5 901 No protein Retained intron - - - TSL:3,
COL9A3-207 ENST00000469802.5 680 No protein Retained intron - - - TSL:2,
COL9A3-211 ENST00000481800.1 548 No protein Retained intron - - - TSL:4,
COL9A3-213 ENST00000490398.5 496 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene COL9A3
This KO Strategy loading
Red Cotton™ Notes Gene COL9A3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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