CRISPR-U™ Gene Knockout Cell Line Strategy

COL6A2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human COL6A2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol COL6A2
Gene id 1292
Organism Homo sapiens
Official full symbol collagen type VI alpha 2 chain
Gene type protein-coding
Also known as BTHLM1, PP3610, UCMD1
Summary This gene encodes one of the three alpha chains of type VI collagen, a beaded filament collagen found in most connective tissues. The product of this gene contains several domains similar to von Willebrand Factor type A domains. These domains have been shown to bind extracellular matrix proteins, an interaction that explains the importance of this collagen in organizing matrix components. Mutations in this gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene.
Genomic regions Chromosome 21
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
COL6A2-202 ENST00000310645.9 3446 828aa Protein coding CCDS13730 P12110-3 - TSL:5, GENCODE basic,
COL6A2-201 ENST00000300527.9 3445 1019aa Protein coding CCDS13728 P12110-1 NM_001849.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
COL6A2-204 ENST00000409416.6 3367 828aa Protein coding CCDS13730 P12110-3 - TSL:5, GENCODE basic,
COL6A2-203 ENST00000397763.5 3101 918aa Protein coding CCDS13729 P12110-2 - TSL:5, GENCODE basic,
COL6A2-206 ENST00000436769.5 919 180aa Protein coding - C9JH44 - CDS 3' incomplete, TSL:2,
COL6A2-205 ENST00000413758.1 750 250aa Protein coding - H7C0M5 - CDS 5' and 3' incomplete, TSL:3,
COL6A2-207 ENST00000460886.1 306 No protein Processed transcript - - - TSL:2,
COL6A2-208 ENST00000485591.1 777 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene COL6A2
This KO Strategy loading
Red Cotton™ Notes Gene COL6A2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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