CRISPR-U™ Gene Knockout Cell Line
Strategy
COL6A2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human COL6A2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | COL6A2 |
| Gene id | 1292 |
| Organism | Homo sapiens |
| Official full symbol | collagen type VI alpha 2 chain |
| Gene type | protein-coding |
| Also known as | BTHLM1, PP3610, UCMD1 |
| Summary | This gene encodes one of the three alpha chains of type VI collagen, a beaded filament collagen found in most connective tissues. The product of this gene contains several domains similar to von Willebrand Factor type A domains. These domains have been shown to bind extracellular matrix proteins, an interaction that explains the importance of this collagen in organizing matrix components. Mutations in this gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene. |
| Genomic regions | Chromosome 21 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| COL6A2-202 | ENST00000310645.9 | 3446 | 828aa | Protein coding | CCDS13730 | P12110-3 | - | TSL:5, GENCODE basic, |
| COL6A2-201 | ENST00000300527.9 | 3445 | 1019aa | Protein coding | CCDS13728 | P12110-1 | NM_001849.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| COL6A2-204 | ENST00000409416.6 | 3367 | 828aa | Protein coding | CCDS13730 | P12110-3 | - | TSL:5, GENCODE basic, |
| COL6A2-203 | ENST00000397763.5 | 3101 | 918aa | Protein coding | CCDS13729 | P12110-2 | - | TSL:5, GENCODE basic, |
| COL6A2-206 | ENST00000436769.5 | 919 | 180aa | Protein coding | - | C9JH44 | - | CDS 3' incomplete, TSL:2, |
| COL6A2-205 | ENST00000413758.1 | 750 | 250aa | Protein coding | - | H7C0M5 | - | CDS 5' and 3' incomplete, TSL:3, |
| COL6A2-207 | ENST00000460886.1 | 306 | No protein | Processed transcript | - | - | - | TSL:2, |
| COL6A2-208 | ENST00000485591.1 | 777 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | COL6A2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene COL6A2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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