CRISPR-U™ Gene Knockout Cell Line Strategy

COL5A1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human COL5A1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol COL5A1
Gene id 1289
Organism Homo sapiens
Official full symbol collagen type V alpha 1 chain
Gene type protein-coding
Also known as EDSC, EDSCL1
Summary This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants.
Genomic regions Chromosome 9
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
COL5A1-201 ENST00000371817.8 8442 1838aa Protein coding CCDS6982 P20908-1 NM_000093.5 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
COL5A1-208 ENST00000618395.4 8437 1838aa Protein coding CCDS75932 P20908-2 - TSL:5, GENCODE basic, APPRIS ALT2,
COL5A1-202 ENST00000371820.3 628 210aa Protein coding - H7BY82 - CDS 5' and 3' incomplete, TSL:2,
COL5A1-205 ENST00000464187.1 1830 No protein Processed transcript - - - TSL:2,
COL5A1-207 ENST00000469093.1 670 No protein Processed transcript - - - TSL:3,
COL5A1-204 ENST00000463925.1 533 No protein Processed transcript - - - TSL:3,
COL5A1-203 ENST00000460264.5 913 No protein Retained intron - - - TSL:3,
COL5A1-206 ENST00000465877.1 583 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene COL5A1
This KO Strategy loading
Red Cotton™ Notes Gene COL5A1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×