CRISPR-U™ Gene Knockout Cell Line
Strategy
COL5A1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human COL5A1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | COL5A1 |
| Gene id | 1289 |
| Organism | Homo sapiens |
| Official full symbol | collagen type V alpha 1 chain |
| Gene type | protein-coding |
| Also known as | EDSC, EDSCL1 |
| Summary | This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| COL5A1-201 | ENST00000371817.8 | 8442 | 1838aa | Protein coding | CCDS6982 | P20908-1 | NM_000093.5 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| COL5A1-208 | ENST00000618395.4 | 8437 | 1838aa | Protein coding | CCDS75932 | P20908-2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| COL5A1-202 | ENST00000371820.3 | 628 | 210aa | Protein coding | - | H7BY82 | - | CDS 5' and 3' incomplete, TSL:2, |
| COL5A1-205 | ENST00000464187.1 | 1830 | No protein | Processed transcript | - | - | - | TSL:2, |
| COL5A1-207 | ENST00000469093.1 | 670 | No protein | Processed transcript | - | - | - | TSL:3, |
| COL5A1-204 | ENST00000463925.1 | 533 | No protein | Processed transcript | - | - | - | TSL:3, |
| COL5A1-203 | ENST00000460264.5 | 913 | No protein | Retained intron | - | - | - | TSL:3, |
| COL5A1-206 | ENST00000465877.1 | 583 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | COL5A1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene COL5A1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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