CRISPR-U™ Gene Knockout Cell Line Strategy

COL4A6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human COL4A6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol COL4A6
Gene id 1288
Organism Homo sapiens
Official full symbol collagen type IV alpha 6 chain
Gene type protein-coding
Also known as CXDELq22.3, DELXq22.3, DFNX6
Summary This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Alternative splicing results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome X
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
COL4A6-203 ENST00000394872.6 6750 1707aa Protein coding CCDS76010 A8MXH5 - TSL:5, GENCODE basic, APPRIS P1,
COL4A6-201 ENST00000334504.12 6685 1690aa Protein coding CCDS14542 Q14031-2 NM_033641.4 TSL:5, GENCODE basic, MANE Select v0.92,
COL4A6-210 ENST00000621266.4 6627 1666aa Protein coding CCDS76009 A0A087WZY5 - TSL:1, GENCODE basic,
COL4A6-202 ENST00000372216.8 6570 1691aa Protein coding CCDS14541 Q14031-1 - TSL:1, GENCODE basic,
COL4A6-208 ENST00000538570.5 6528 1633aa Protein coding CCDS76008 F5H3Q5 - TSL:1, GENCODE basic,
COL4A6-209 ENST00000545689.2 6380 1678aa Protein coding - F5H851 - TSL:1, GENCODE basic,
COL4A6-204 ENST00000461897.5 1889 No protein Processed transcript - - - TSL:1,
COL4A6-206 ENST00000477085.1 1784 No protein Processed transcript - - - TSL:1,
COL4A6-207 ENST00000487645.1 812 No protein Processed transcript - - - TSL:3,
COL4A6-205 ENST00000468338.5 865 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene COL4A6
This KO Strategy loading
Red Cotton™ Notes Gene COL4A6 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×

Comment: