CRISPR-U™ Gene Knockout Cell Line Strategy

COL4A5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human COL4A5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol COL4A5
Gene id 1287
Organism Homo sapiens
Official full symbol collagen type IV alpha 5 chain
Gene type protein-coding
Also known as ASLN, ATS, ATS1, CA54
Summary This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene.
Genomic regions Chromosome X
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
COL4A5-201 ENST00000328300.11 6531 1691aa Protein coding CCDS35366 P29400-2 NM_033380.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
COL4A5-202 ENST00000361603.7 6427 1685aa Protein coding CCDS14543 P29400-1 - TSL:2, GENCODE basic, APPRIS ALT2,
COL4A5-205 ENST00000483338.1 3622 695aa Protein coding - Q49AM6 - TSL:1, GENCODE basic,
COL4A5-207 ENST00000504541.1 859 73aa Protein coding - H0Y9R8 - CDS 5' incomplete, TSL:5,
COL4A5-210 ENST00000515658.1 790 115aa Protein coding - H0Y998 - CDS 5' incomplete, TSL:5,
COL4A5-208 ENST00000505728.1 537 174aa Protein coding - H0Y9H0 - CDS 5' incomplete, TSL:3,
COL4A5-211 ENST00000642185.1 1014 40aa Nonsense mediated decay - A0A2R8Y5W0 - -
COL4A5-204 ENST00000477429.1 564 No protein Processed transcript - - - TSL:1,
COL4A5-212 ENST00000644079.1 3915 No protein Retained intron - - - -
COL4A5-209 ENST00000510690.2 1581 No protein Retained intron - - - TSL:4,
COL4A5-203 ENST00000470339.1 782 No protein Retained intron - - - TSL:3,
COL4A5-206 ENST00000489230.1 737 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene COL4A5
This KO Strategy loading
Red Cotton™ Notes Gene COL4A5 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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