CRISPR-U™ Gene Knockout Cell Line
Strategy
COL4A2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human COL4A2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | COL4A2 |
| Gene id | 1284 |
| Organism | Homo sapiens |
| Official full symbol | collagen type IV alpha 2 chain |
| Gene type | protein-coding |
| Also known as | BSVD2, ICH, POREN2 |
| Summary | This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. |
| Genomic regions | Chromosome 13 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| COL4A2-201 | ENST00000360467.7 | 6446 | 1712aa | Protein coding | CCDS41907 | A0A024RDW8 P08572 | NM_001846.4 | TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| COL4A2-213 | ENST00000649101.1 | 5158 | 79aa | Protein coding | - | A0A3B3ITN7 | - | GENCODE basic, |
| COL4A2-218 | ENST00000650540.1 | 3108 | 361aa | Protein coding | - | A0A3B3ITQ8 | - | GENCODE basic, |
| COL4A2-210 | ENST00000617564.2 | 1690 | 311aa | Protein coding | - | A0A087WY39 | - | CDS 5' incomplete, TSL:NA, |
| COL4A2-211 | ENST00000619688.2 | 1488 | 93aa | Protein coding | - | A0A3B3IRV2 | - | CDS 5' incomplete, TSL:NA, |
| COL4A2-206 | ENST00000480609.1 | 535 | 166aa | Protein coding | - | A0A3B3IS47 | - | CDS 5' incomplete, TSL:2, |
| COL4A2-202 | ENST00000400163.7 | 484 | 102aa | Protein coding | - | A2A352 | - | CDS 3' incomplete, TSL:5, |
| COL4A2-209 | ENST00000494852.2 | 473 | 121aa | Protein coding | - | A0A3B3IT80 | - | CDS 5' incomplete, TSL:3, |
| COL4A2-215 | ENST00000649951.1 | 466 | 47aa | Protein coding | - | A0A3B3IS04 | - | CDS 5' incomplete, |
| COL4A2-204 | ENST00000463084.1 | 679 | No protein | Processed transcript | - | - | - | TSL:3, |
| COL4A2-207 | ENST00000480771.5 | 585 | No protein | Processed transcript | - | - | - | TSL:4, |
| COL4A2-217 | ENST00000650322.1 | 580 | No protein | Processed transcript | - | - | - | - |
| COL4A2-216 | ENST00000650225.1 | 3630 | No protein | Retained intron | - | - | - | - |
| COL4A2-208 | ENST00000483683.2 | 1980 | No protein | Retained intron | - | - | - | TSL:2, |
| COL4A2-212 | ENST00000648222.1 | 1663 | No protein | Retained intron | - | - | - | - |
| COL4A2-203 | ENST00000462309.2 | 849 | No protein | Retained intron | - | - | - | TSL:3, |
| COL4A2-214 | ENST00000649396.1 | 517 | No protein | Retained intron | - | - | - | - |
| COL4A2-205 | ENST00000478681.1 | 337 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | COL4A2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene COL4A2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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