CRISPR-U™ Gene Knockout Cell Line Strategy

C1orf131 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human C1orf131 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol C1orf131
Gene id 128061
Organism Homo sapiens
Official full symbol chromosome 1 open reading frame 131
Gene type protein-coding
Genomic regions Chromosome 1
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
C1orf131-203 ENST00000366651.7 1432 292aa Protein coding CCDS73049 Q8NDD1-6 - TSL:1, GENCODE basic, APPRIS ALT2,
C1orf131-202 ENST00000366649.7 1430 293aa Protein coding CCDS1591 Q8NDD1-1 NM_152379.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
C1orf131-201 ENST00000318906.6 2483 249aa Protein coding - Q5TBH9 - TSL:1, GENCODE basic, APPRIS ALT2,
C1orf131-204 ENST00000451322.1 795 256aa Protein coding - Q5TBI2 - CDS 5' incomplete, TSL:5,
C1orf131-206 ENST00000471936.1 2657 No protein Processed transcript - - - TSL:2,
C1orf131-208 ENST00000495795.1 596 No protein Processed transcript - - - TSL:5,
C1orf131-205 ENST00000462669.1 552 No protein Processed transcript - - - TSL:2,
C1orf131-207 ENST00000486384.5 400 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene C1orf131
This KO Strategy loading
Red Cotton™ Notes Gene C1orf131 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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