CRISPR-U™ Gene Knockout Cell Line
Strategy
CNR1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CNR1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CNR1 |
| Gene id | 1268 |
| Organism | Homo sapiens |
| Official full symbol | cannabinoid receptor 1 |
| Gene type | protein-coding |
| Also known as | CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR |
| Summary | This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CNR1-203 | ENST00000369501.2 | 6031 | 472aa | Protein coding | CCDS5015 | P21554-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CNR1-202 | ENST00000369499.2 | 5549 | 472aa | Protein coding | CCDS5015 | P21554-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| CNR1-206 | ENST00000549890.1 | 2122 | 472aa | Protein coding | CCDS5015 | P21554-1 | - | TSL:NA, GENCODE basic, APPRIS P1, |
| CNR1-204 | ENST00000428600.2 | 1550 | 472aa | Protein coding | CCDS5015 | P21554-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CNR1-205 | ENST00000468898.1 | 1320 | 439aa | Protein coding | CCDS5016 | P21554-3 | - | TSL:1, GENCODE basic, |
| CNR1-201 | ENST00000362094.5 | 1252 | 36aa | Protein coding | - | F8W908 | - | TSL:1, GENCODE basic, |
| CNR1-207 | ENST00000551417.1 | 591 | 78aa | Protein coding | - | F8W187 | - | CDS 3' incomplete, TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CNR1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CNR1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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