CRISPR-U™ Gene Knockout Cell Line Strategy

CNR1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CNR1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CNR1
Gene id 1268
Organism Homo sapiens
Official full symbol cannabinoid receptor 1
Gene type protein-coding
Also known as CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR
Summary This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.
Genomic regions Chromosome 6
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CNR1-203 ENST00000369501.2 6031 472aa Protein coding CCDS5015 P21554-1 - TSL:1, GENCODE basic, APPRIS P1,
CNR1-202 ENST00000369499.2 5549 472aa Protein coding CCDS5015 P21554-1 - TSL:5, GENCODE basic, APPRIS P1,
CNR1-206 ENST00000549890.1 2122 472aa Protein coding CCDS5015 P21554-1 - TSL:NA, GENCODE basic, APPRIS P1,
CNR1-204 ENST00000428600.2 1550 472aa Protein coding CCDS5015 P21554-1 - TSL:1, GENCODE basic, APPRIS P1,
CNR1-205 ENST00000468898.1 1320 439aa Protein coding CCDS5016 P21554-3 - TSL:1, GENCODE basic,
CNR1-201 ENST00000362094.5 1252 36aa Protein coding - F8W908 - TSL:1, GENCODE basic,
CNR1-207 ENST00000551417.1 591 78aa Protein coding - F8W187 - CDS 3' incomplete, TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CNR1
This KO Strategy loading
Red Cotton™ Notes Gene CNR1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×