CRISPR-U™ Gene Knockout Cell Line
Strategy
MISP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MISP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MISP |
| Gene id | 126353 |
| Organism | Homo sapiens |
| Official full symbol | mitotic spindle positioning |
| Gene type | protein-coding |
| Also known as | C19orf21, MISP1 |
| Summary | The protein encoded by this gene is an actin-bundling protein involved in determining cell morphology and mitotic progression. The encoded protein is required for the proper positioning of the mitotic spindle. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MISP-201 | ENST00000215582.8 | 2885 | 679aa | Protein coding | CCDS12042 | Q8IVT2 | NM_173481.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MISP-202 | ENST00000614180.1 | 890 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MISP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MISP had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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