CRISPR-U™ Gene Knockout Cell Line
Strategy
NDUFA11 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NDUFA11 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NDUFA11 |
| Gene id | 126328 |
| Organism | Homo sapiens |
| Official full symbol | NADH:ubiquinone oxidoreductase subunit A11 |
| Gene type | protein-coding |
| Also known as | B14.7, CI-B14.7, MC1DN14 |
| Summary | This gene encodes a subunit of the membrane-bound mitochondrial complex I. Complex I is composed of numerous subunits and functions as the NADH-ubiquinol reductase of the mitochondrial electron transport chain. Mutations in this gene are associated with severe mitochondrial complex I deficiency. Alternate splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NDUFA11-202 | ENST00000418389.6 | 2406 | 228aa | Protein coding | CCDS54203 | Q86Y39-2 | - | TSL:2, GENCODE basic, |
| NDUFA11-201 | ENST00000308961.5 | 575 | 141aa | Protein coding | CCDS12155 | Q86Y39-1 | NM_175614.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NDUFA11-205 | ENST00000592634.5 | 2154 | 120aa | Protein coding | - | K7EQ77 | - | TSL:2, GENCODE basic, |
| NDUFA11-207 | ENST00000650663.1 | 1786 | No protein | Processed transcript | - | - | - | - |
| NDUFA11-206 | ENST00000593233.1 | 657 | No protein | Processed transcript | - | - | - | TSL:3, |
| NDUFA11-203 | ENST00000591160.1 | 604 | No protein | Processed transcript | - | - | - | TSL:3, |
| NDUFA11-204 | ENST00000591341.1 | 403 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NDUFA11 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NDUFA11 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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