CRISPR-U™ Gene Knockout Cell Line Strategy

NDUFA11 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NDUFA11 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NDUFA11
Gene id 126328
Organism Homo sapiens
Official full symbol NADH:ubiquinone oxidoreductase subunit A11
Gene type protein-coding
Also known as B14.7, CI-B14.7, MC1DN14
Summary This gene encodes a subunit of the membrane-bound mitochondrial complex I. Complex I is composed of numerous subunits and functions as the NADH-ubiquinol reductase of the mitochondrial electron transport chain. Mutations in this gene are associated with severe mitochondrial complex I deficiency. Alternate splicing results in multiple transcript variants.
Genomic regions Chromosome 19
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NDUFA11-202 ENST00000418389.6 2406 228aa Protein coding CCDS54203 Q86Y39-2 - TSL:2, GENCODE basic,
NDUFA11-201 ENST00000308961.5 575 141aa Protein coding CCDS12155 Q86Y39-1 NM_175614.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NDUFA11-205 ENST00000592634.5 2154 120aa Protein coding - K7EQ77 - TSL:2, GENCODE basic,
NDUFA11-207 ENST00000650663.1 1786 No protein Processed transcript - - - -
NDUFA11-206 ENST00000593233.1 657 No protein Processed transcript - - - TSL:3,
NDUFA11-203 ENST00000591160.1 604 No protein Processed transcript - - - TSL:3,
NDUFA11-204 ENST00000591341.1 403 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NDUFA11
This KO Strategy loading
Red Cotton™ Notes Gene NDUFA11 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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