CRISPR-U™ Gene Knockout Cell Line
Strategy
PAQR4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PAQR4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PAQR4 |
| Gene id | 124222 |
| Organism | Homo sapiens |
| Official full symbol | progestin and adipoQ receptor family member 4 |
| Gene type | protein-coding |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PAQR4-202 | ENST00000318782.9 | 2685 | 273aa | Protein coding | CCDS10485 | Q8N4S7-1 | NM_152341.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PAQR4-201 | ENST00000293978.12 | 2644 | 234aa | Protein coding | CCDS66911 | Q8N4S7-2 | - | TSL:2, GENCODE basic, |
| PAQR4-203 | ENST00000572687.1 | 2251 | 199aa | Protein coding | CCDS66912 | I3L265 | - | TSL:2, GENCODE basic, |
| PAQR4-204 | ENST00000574988.1 | 869 | 206aa | Protein coding | CCDS73814 | I3L1A2 | - | TSL:2, GENCODE basic, |
| PAQR4-205 | ENST00000576565.1 | 842 | 206aa | Protein coding | CCDS73814 | I3L1A2 | - | TSL:2, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PAQR4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PAQR4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: