CRISPR-U™ Gene Knockout Cell Line
Strategy
CLTA Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CLTA Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CLTA |
| Gene id | 1211 |
| Organism | Homo sapiens |
| Official full symbol | clathrin light chain A |
| Gene type | protein-coding |
| Also known as | LCA |
| Summary | Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CLTA-201 | ENST00000242285.10 | 1152 | 248aa | Protein coding | CCDS6601 | P09496-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| CLTA-207 | ENST00000470744.5 | 1102 | 230aa | Protein coding | CCDS55306 | P09496-4 | - | TSL:2, GENCODE basic, |
| CLTA-203 | ENST00000396603.6 | 1090 | 236aa | Protein coding | CCDS43802 | P09496-3 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| CLTA-202 | ENST00000345519.10 | 1078 | 218aa | Protein coding | CCDS6600 | P09496-2 | NM_001833.4 | TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| CLTA-210 | ENST00000540080.5 | 989 | 166aa | Protein coding | CCDS55307 | P09496-5 | - | TSL:2, GENCODE basic, |
| CLTA-206 | ENST00000466396.5 | 695 | 196aa | Protein coding | CCDS83364 | C9J8P9 | - | TSL:2, GENCODE basic, |
| CLTA-204 | ENST00000433436.6 | 1235 | 248aa | Protein coding | - | P09496-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| CLTA-209 | ENST00000538225.5 | 1181 | 230aa | Protein coding | - | P09496-4 | - | TSL:2, GENCODE basic, |
| CLTA-205 | ENST00000464497.5 | 1890 | 260aa | Nonsense mediated decay | - | F8WF69 | - | TSL:5, |
| CLTA-208 | ENST00000493185.2 | 714 | 64aa | Nonsense mediated decay | - | S4R3F6 | - | CDS 5' incomplete, TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CLTA |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CLTA had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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