CRISPR-U™ Gene Knockout Cell Line Strategy

CLTA Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CLTA Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CLTA
Gene id 1211
Organism Homo sapiens
Official full symbol clathrin light chain A
Gene type protein-coding
Also known as LCA
Summary Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12.
Genomic regions Chromosome 9
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CLTA-201 ENST00000242285.10 1152 248aa Protein coding CCDS6601 P09496-1 - TSL:1, GENCODE basic, APPRIS P4,
CLTA-207 ENST00000470744.5 1102 230aa Protein coding CCDS55306 P09496-4 - TSL:2, GENCODE basic,
CLTA-203 ENST00000396603.6 1090 236aa Protein coding CCDS43802 P09496-3 - TSL:1, GENCODE basic, APPRIS ALT1,
CLTA-202 ENST00000345519.10 1078 218aa Protein coding CCDS6600 P09496-2 NM_001833.4 TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
CLTA-210 ENST00000540080.5 989 166aa Protein coding CCDS55307 P09496-5 - TSL:2, GENCODE basic,
CLTA-206 ENST00000466396.5 695 196aa Protein coding CCDS83364 C9J8P9 - TSL:2, GENCODE basic,
CLTA-204 ENST00000433436.6 1235 248aa Protein coding - P09496-1 - TSL:1, GENCODE basic, APPRIS P4,
CLTA-209 ENST00000538225.5 1181 230aa Protein coding - P09496-4 - TSL:2, GENCODE basic,
CLTA-205 ENST00000464497.5 1890 260aa Nonsense mediated decay - F8WF69 - TSL:5,
CLTA-208 ENST00000493185.2 714 64aa Nonsense mediated decay - S4R3F6 - CDS 5' incomplete, TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CLTA
This KO Strategy loading
Red Cotton™ Notes Gene CLTA had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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