CRISPR-U™ Gene Knockout Cell Line Strategy

CLK3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CLK3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CLK3
Gene id 1198
Organism Homo sapiens
Official full symbol CDC like kinase 3
Gene type protein-coding
Also known as PHCLK3, PHCLK3/152
Summary This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Two transcript variants encoding different isoforms have been found for this gene. Related pseudogenes are located on chromosomes 1 and 9.
Genomic regions Chromosome 15
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CLK3-202 ENST00000395066.8 2621 490aa Protein coding CCDS10265 P49761-4 - TSL:1, GENCODE basic, APPRIS P1,
CLK3-201 ENST00000345005.8 1846 490aa Protein coding CCDS10265 P49761-1 - TSL:1, GENCODE basic, APPRIS P1,
CLK3-212 ENST00000563418.2 10498 70aa Protein coding - H3BRW2 - CDS 5' incomplete, TSL:3,
CLK3-221 ENST00000568139.6 918 286aa Protein coding - H3BQG1 - CDS 3' incomplete, TSL:5,
CLK3-213 ENST00000563842.5 860 190aa Protein coding - H3BRE4 - CDS 3' incomplete, TSL:5,
CLK3-214 ENST00000564096.1 805 239aa Protein coding - H3BVF8 - CDS 3' incomplete, TSL:2,
CLK3-217 ENST00000566126.5 732 224aa Protein coding - H3BUL5 - CDS 3' incomplete, TSL:5,
CLK3-218 ENST00000566926.1 646 193aa Protein coding - H3BNQ5 - CDS 5' incomplete, TSL:5,
CLK3-207 ENST00000562389.5 572 117aa Protein coding - H3BRT8 - CDS 3' incomplete, TSL:4,
CLK3-209 ENST00000562670.5 544 145aa Protein coding - H3BQR7 - CDS 3' incomplete, TSL:4,
CLK3-211 ENST00000563297.5 526 19aa Protein coding - H3BTW9 - CDS 3' incomplete, TSL:4,
CLK3-223 ENST00000568488.6 481 145aa Protein coding - H3BV35 - CDS 3' incomplete, TSL:3,
CLK3-204 ENST00000483723.5 1749 152aa Nonsense mediated decay - P49761-2 - TSL:2,
CLK3-216 ENST00000564468.5 929 59aa Nonsense mediated decay - H3BNC8 - CDS 5' incomplete, TSL:3,
CLK3-225 ENST00000569063.5 833 173aa Nonsense mediated decay - H3BRK0 - CDS 5' incomplete, TSL:5,
CLK3-215 ENST00000564353.5 2847 No protein Processed transcript - - - TSL:2,
CLK3-205 ENST00000561673.1 639 No protein Processed transcript - - - TSL:3,
CLK3-220 ENST00000567992.1 603 No protein Processed transcript - - - TSL:2,
CLK3-219 ENST00000567805.5 327 No protein Processed transcript - - - TSL:3,
CLK3-203 ENST00000454830.6 3787 No protein Retained intron - - - TSL:5,
CLK3-226 ENST00000569406.5 3591 No protein Retained intron - - - TSL:2,
CLK3-206 ENST00000562078.5 2455 No protein Retained intron - - - TSL:2,
CLK3-224 ENST00000568605.5 1047 No protein Retained intron - - - TSL:2,
CLK3-222 ENST00000568232.1 958 No protein Retained intron - - - TSL:NA,
CLK3-227 ENST00000570296.1 916 No protein Retained intron - - - TSL:5,
CLK3-208 ENST00000562626.1 812 No protein Retained intron - - - TSL:2,
CLK3-210 ENST00000563112.1 665 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CLK3
This KO Strategy loading
Red Cotton™ Notes Gene CLK3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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