CRISPR-U™ Gene Knockout Cell Line
Strategy
CLK3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CLK3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CLK3 |
| Gene id | 1198 |
| Organism | Homo sapiens |
| Official full symbol | CDC like kinase 3 |
| Gene type | protein-coding |
| Also known as | PHCLK3, PHCLK3/152 |
| Summary | This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Two transcript variants encoding different isoforms have been found for this gene. Related pseudogenes are located on chromosomes 1 and 9. |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CLK3-202 | ENST00000395066.8 | 2621 | 490aa | Protein coding | CCDS10265 | P49761-4 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CLK3-201 | ENST00000345005.8 | 1846 | 490aa | Protein coding | CCDS10265 | P49761-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CLK3-212 | ENST00000563418.2 | 10498 | 70aa | Protein coding | - | H3BRW2 | - | CDS 5' incomplete, TSL:3, |
| CLK3-221 | ENST00000568139.6 | 918 | 286aa | Protein coding | - | H3BQG1 | - | CDS 3' incomplete, TSL:5, |
| CLK3-213 | ENST00000563842.5 | 860 | 190aa | Protein coding | - | H3BRE4 | - | CDS 3' incomplete, TSL:5, |
| CLK3-214 | ENST00000564096.1 | 805 | 239aa | Protein coding | - | H3BVF8 | - | CDS 3' incomplete, TSL:2, |
| CLK3-217 | ENST00000566126.5 | 732 | 224aa | Protein coding | - | H3BUL5 | - | CDS 3' incomplete, TSL:5, |
| CLK3-218 | ENST00000566926.1 | 646 | 193aa | Protein coding | - | H3BNQ5 | - | CDS 5' incomplete, TSL:5, |
| CLK3-207 | ENST00000562389.5 | 572 | 117aa | Protein coding | - | H3BRT8 | - | CDS 3' incomplete, TSL:4, |
| CLK3-209 | ENST00000562670.5 | 544 | 145aa | Protein coding | - | H3BQR7 | - | CDS 3' incomplete, TSL:4, |
| CLK3-211 | ENST00000563297.5 | 526 | 19aa | Protein coding | - | H3BTW9 | - | CDS 3' incomplete, TSL:4, |
| CLK3-223 | ENST00000568488.6 | 481 | 145aa | Protein coding | - | H3BV35 | - | CDS 3' incomplete, TSL:3, |
| CLK3-204 | ENST00000483723.5 | 1749 | 152aa | Nonsense mediated decay | - | P49761-2 | - | TSL:2, |
| CLK3-216 | ENST00000564468.5 | 929 | 59aa | Nonsense mediated decay | - | H3BNC8 | - | CDS 5' incomplete, TSL:3, |
| CLK3-225 | ENST00000569063.5 | 833 | 173aa | Nonsense mediated decay | - | H3BRK0 | - | CDS 5' incomplete, TSL:5, |
| CLK3-215 | ENST00000564353.5 | 2847 | No protein | Processed transcript | - | - | - | TSL:2, |
| CLK3-205 | ENST00000561673.1 | 639 | No protein | Processed transcript | - | - | - | TSL:3, |
| CLK3-220 | ENST00000567992.1 | 603 | No protein | Processed transcript | - | - | - | TSL:2, |
| CLK3-219 | ENST00000567805.5 | 327 | No protein | Processed transcript | - | - | - | TSL:3, |
| CLK3-203 | ENST00000454830.6 | 3787 | No protein | Retained intron | - | - | - | TSL:5, |
| CLK3-226 | ENST00000569406.5 | 3591 | No protein | Retained intron | - | - | - | TSL:2, |
| CLK3-206 | ENST00000562078.5 | 2455 | No protein | Retained intron | - | - | - | TSL:2, |
| CLK3-224 | ENST00000568605.5 | 1047 | No protein | Retained intron | - | - | - | TSL:2, |
| CLK3-222 | ENST00000568232.1 | 958 | No protein | Retained intron | - | - | - | TSL:NA, |
| CLK3-227 | ENST00000570296.1 | 916 | No protein | Retained intron | - | - | - | TSL:5, |
| CLK3-208 | ENST00000562626.1 | 812 | No protein | Retained intron | - | - | - | TSL:2, |
| CLK3-210 | ENST00000563112.1 | 665 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CLK3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CLK3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: