CRISPR-U™ Gene Knockout Cell Line
Strategy
CLK2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CLK2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CLK2 |
| Gene id | 1196 |
| Organism | Homo sapiens |
| Official full symbol | CDC like kinase 2 |
| Gene type | protein-coding |
| Summary | This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CLK2-203 | ENST00000368361.9 | 2154 | 499aa | Protein coding | CCDS72939 | P49760-1 | NM_001294338.2 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| CLK2-201 | ENST00000355560.4 | 2120 | 497aa | Protein coding | CCDS86020 | B1AVT0 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| CLK2-202 | ENST00000361168.9 | 1934 | 498aa | Protein coding | CCDS1107 | P49760-3 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| CLK2-207 | ENST00000497188.1 | 2702 | No protein | Processed transcript | - | - | - | TSL:2, |
| CLK2-205 | ENST00000476983.5 | 1885 | No protein | Processed transcript | - | - | - | TSL:1, |
| CLK2-204 | ENST00000471047.1 | 701 | No protein | Processed transcript | - | - | - | TSL:3, |
| CLK2-206 | ENST00000484699.1 | 585 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CLK2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CLK2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: