CRISPR-U™ Gene Knockout Cell Line Strategy

CLK2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CLK2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CLK2
Gene id 1196
Organism Homo sapiens
Official full symbol CDC like kinase 2
Gene type protein-coding
Summary This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CLK2-203 ENST00000368361.9 2154 499aa Protein coding CCDS72939 P49760-1 NM_001294338.2 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CLK2-201 ENST00000355560.4 2120 497aa Protein coding CCDS86020 B1AVT0 - TSL:5, GENCODE basic, APPRIS ALT1,
CLK2-202 ENST00000361168.9 1934 498aa Protein coding CCDS1107 P49760-3 - TSL:1, GENCODE basic, APPRIS ALT1,
CLK2-207 ENST00000497188.1 2702 No protein Processed transcript - - - TSL:2,
CLK2-205 ENST00000476983.5 1885 No protein Processed transcript - - - TSL:1,
CLK2-204 ENST00000471047.1 701 No protein Processed transcript - - - TSL:3,
CLK2-206 ENST00000484699.1 585 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CLK2
This KO Strategy loading
Red Cotton™ Notes Gene CLK2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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