CRISPR-U™ Gene Knockout Cell Line
Strategy
CLK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CLK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CLK1 |
| Gene id | 1195 |
| Organism | Homo sapiens |
| Official full symbol | CDC like kinase 1 |
| Gene type | protein-coding |
| Also known as | CLK, CLK/STY, STY |
| Summary | This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CLK1-205 | ENST00000434813.3 | 2026 | 526aa | Protein coding | CCDS54427 | P49759-3 | - | TSL:2, GENCODE basic, |
| CLK1-201 | ENST00000321356.9 | 1847 | 484aa | Protein coding | CCDS2331 | P49759-1 | NM_004071.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CLK1-215 | ENST00000621181.4 | 1843 | 454aa | Protein coding | - | A0A087WVK0 | - | TSL:1, GENCODE basic, |
| CLK1-203 | ENST00000409769.6 | 1192 | 307aa | Protein coding | - | B8ZZR0 | - | TSL:2, GENCODE basic, |
| CLK1-204 | ENST00000432425.5 | 1682 | 136aa | Nonsense mediated decay | - | P49759-2 | - | TSL:1, |
| CLK1-202 | ENST00000409403.6 | 1664 | 355aa | Nonsense mediated decay | - | F8WBF5 | - | TSL:5, |
| CLK1-213 | ENST00000492793.1 | 596 | No protein | Processed transcript | - | - | - | TSL:4, |
| CLK1-210 | ENST00000473565.5 | 3208 | No protein | Retained intron | - | - | - | TSL:1, |
| CLK1-207 | ENST00000461981.5 | 3173 | No protein | Retained intron | - | - | - | TSL:2, |
| CLK1-211 | ENST00000481641.1 | 690 | No protein | Retained intron | - | - | - | TSL:4, |
| CLK1-212 | ENST00000482590.1 | 626 | No protein | Retained intron | - | - | - | TSL:2, |
| CLK1-208 | ENST00000464454.1 | 484 | No protein | Retained intron | - | - | - | TSL:2, |
| CLK1-214 | ENST00000496205.1 | 480 | No protein | Retained intron | - | - | - | TSL:2, |
| CLK1-209 | ENST00000472679.1 | 461 | No protein | Retained intron | - | - | - | TSL:2, |
| CLK1-206 | ENST00000461326.1 | 435 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CLK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CLK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: