CRISPR-U™ Gene Knockout Cell Line Strategy

CLK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CLK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CLK1
Gene id 1195
Organism Homo sapiens
Official full symbol CDC like kinase 1
Gene type protein-coding
Also known as CLK, CLK/STY, STY
Summary This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CLK1-205 ENST00000434813.3 2026 526aa Protein coding CCDS54427 P49759-3 - TSL:2, GENCODE basic,
CLK1-201 ENST00000321356.9 1847 484aa Protein coding CCDS2331 P49759-1 NM_004071.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CLK1-215 ENST00000621181.4 1843 454aa Protein coding - A0A087WVK0 - TSL:1, GENCODE basic,
CLK1-203 ENST00000409769.6 1192 307aa Protein coding - B8ZZR0 - TSL:2, GENCODE basic,
CLK1-204 ENST00000432425.5 1682 136aa Nonsense mediated decay - P49759-2 - TSL:1,
CLK1-202 ENST00000409403.6 1664 355aa Nonsense mediated decay - F8WBF5 - TSL:5,
CLK1-213 ENST00000492793.1 596 No protein Processed transcript - - - TSL:4,
CLK1-210 ENST00000473565.5 3208 No protein Retained intron - - - TSL:1,
CLK1-207 ENST00000461981.5 3173 No protein Retained intron - - - TSL:2,
CLK1-211 ENST00000481641.1 690 No protein Retained intron - - - TSL:4,
CLK1-212 ENST00000482590.1 626 No protein Retained intron - - - TSL:2,
CLK1-208 ENST00000464454.1 484 No protein Retained intron - - - TSL:2,
CLK1-214 ENST00000496205.1 480 No protein Retained intron - - - TSL:2,
CLK1-209 ENST00000472679.1 461 No protein Retained intron - - - TSL:2,
CLK1-206 ENST00000461326.1 435 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CLK1
This KO Strategy loading
Red Cotton™ Notes Gene CLK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×