CRISPR-U™ Gene Knockout Cell Line Strategy

CLCN7 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CLCN7 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CLCN7
Gene id 1186
Organism Homo sapiens
Official full symbol chloride voltage-gated channel 7
Gene type protein-coding
Also known as CLC-7, CLC7, HOD, OPTA2, OPTB4, PPP1R63
Summary The product of this gene belongs to the CLC chloride channel family of proteins. Chloride channels play important roles in the plasma membrane and in intracellular organelles. This gene encodes chloride channel 7. Defects in this gene are the cause of osteopetrosis autosomal recessive type 4 (OPTB4), also called infantile malignant osteopetrosis type 2 as well as the cause of autosomal dominant osteopetrosis type 2 (OPTA2), also called autosomal dominant Albers-Schonberg disease or marble disease autosoml dominant. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. OPTA2 is the most common form of osteopetrosis, occurring in adolescence or adulthood.
Genomic regions Chromosome 16
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CLCN7-202 ENST00000382745.9 4168 805aa Protein coding CCDS32361 P51798-1 NM_001287.6 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
CLCN7-203 ENST00000448525.5 4151 781aa Protein coding CCDS45378 P51798-2 - TSL:2, GENCODE basic, APPRIS ALT2,
CLCN7-201 ENST00000262318.12 3717 901aa Protein coding - H0Y2M6 - TSL:5, GENCODE basic,
CLCN7-214 ENST00000569851.6 1014 216aa Protein coding - I3L470 - CDS 3' incomplete, TSL:5,
CLCN7-207 ENST00000564568.1 533 118aa Protein coding - H3BNG8 - CDS 3' incomplete, TSL:4,
CLCN7-210 ENST00000566812.1 434 No protein Processed transcript - - - TSL:4,
CLCN7-205 ENST00000563642.6 4184 No protein Retained intron - - - TSL:2,
CLCN7-209 ENST00000565092.6 2740 No protein Retained intron - - - TSL:2,
CLCN7-206 ENST00000563822.1 851 No protein Retained intron - - - TSL:5,
CLCN7-208 ENST00000564968.1 652 No protein Retained intron - - - TSL:4,
CLCN7-213 ENST00000567836.2 647 No protein Retained intron - - - TSL:2,
CLCN7-211 ENST00000567139.1 581 No protein Retained intron - - - TSL:2,
CLCN7-212 ENST00000567789.1 577 No protein Retained intron - - - TSL:2,
CLCN7-204 ENST00000561665.5 561 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CLCN7
This KO Strategy loading
Red Cotton™ Notes Gene CLCN7 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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